In vivo biofilm formation on different dental ceramics.
In vivo biofilm formation on different dental ceramics.
复制标题
不同牙科陶瓷上的体内生物膜形成。
DOI：
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发表时间：
2011
影响因子：
1.9
通讯作者：
M. Stiesch
中科院分区：
文献类型：
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作者：
Felicia Bremer;S. Grade;P. Kohorst;M. Stiesch
目标
研究口腔生物膜在不同牙科陶瓷材料上的形成情况。
方法和材料
包括五种不同的陶瓷材料：饰面玻璃陶瓷、二硅酸锂玻璃陶瓷、钇稳定的氧化锆（Y-TZP）、热等静压（HIP）Y-TZP陶瓷和具有25%氧化铝的HIP Y-TZP陶瓷。将测试样本连接到单独设计的丙烯酸装置上;五名志愿者在上颌弓中佩戴这些装置24小时。口内暴露后，将样本从装置上取下，并对粘附的生物膜进行活体染色。然后，通过共聚焦激光扫描显微镜测定二维表面涂层和粘附生物膜的厚度。使用单因素ANOVA进行统计分析，显著性水平设定为0.05。
结果
在不同陶瓷材料之间发现细菌表面涂层和生物膜厚度的显著差异（P <0.001）。在HIP Y-TZP陶瓷上测定最低的表面涂层（19.0%）和生物膜厚度（1.9 Μm）;用二硅酸锂玻璃陶瓷确定最高的平均值（46.8%，12.6 Μm）。
结论
不同类型的牙科陶瓷上的生物膜形成有显着差异，特别是氧化锆表现出低菌斑积累。除了其高强度外，低菌斑积累使氧化锆成为一种有前途的材料，用于各种适应症（包括种植基台和套筒冠），以前只能用金属基材料来满足。
OBJECTIVES
To investigate the formation of oral biofilm on various dental ceramics in vivo.
METHOD AND MATERIALS
Five different ceramic materials were included: a veneering glass- ceramic, a lithium disilicate glass-ceramic, a yttrium-stabilized zirconia (Y-TZP), a hot isostatically pressed (HIP) Y-TZP ceramic, and an HIP Y-TZP ceramic with 25% alumina. Test specimens were attached to individually designed acrylic appliances; five volunteers wore these appliances for 24 hours in the maxillary arch. After intraoral exposure, the samples were removed from the appliances and the adhering biofilms vitally stained. Then, the two-dimensional surface coating and thickness of the adhering biofilm were determined by confocal laser scanning microscopy. Statistical analysis was performed using one-way ANOVA with the level of significance set at .05.
RESULTS
Significant differences (P < .001) in the bacterial surface coating and in the thickness of the biofilm were found between the various ceramic materials. The lowest surface coating (19.0%) and biofilm thickness (1.9 Μm) were determined on the HIP Y-TZP ceramic; the highest mean values were identified with the lithium disilicate glass-ceramic (46.8%, 12.6 Μm).
CONCLUSION
Biofilm formation on various types of dental ceramics differed significantly; in particular, zirconia exhibited low plaque accumulation. In addition to its high strength, low plaque accumulation makes zirconia a promising material for various indications (including implant abutments and telescopic crowns) that previously were met only with metal-based materials.