Pathogenesis of hepatitis C virus coinfection in the brains of patients infected with HIV
Pathogenesis of hepatitis C virus coinfection in the brains of patients infected with HIV
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DOI：
10.1086/519285
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发表时间：
2007-08-01
影响因子：
6.4
通讯作者：
Masliah, Eliezer
中科院分区：
文献类型：
--
作者：
Letendre, Scott;Paulino, Amy D.;Masliah, Eliezer
人类免疫缺陷病毒（HIV）累及神经系统仍然是一个严重的问题。在有非法药物使用史的艾滋病毒感染者中，合并感染丙型肝炎病毒（HCV）的人群正在快速增长。然而，很少有研究评估HCV侵入中枢神经系统（CNS）及其对hiv感染者的临床和神经病理学影响。为此，研究了HCV在HIV感染者大脑中的分布。巢式聚合酶链反应检测到CNS中HCV RNA的存在与甲基苯丙胺使用史、大量的死前认知障碍和丰富的星形胶质细胞增生以及较轻的HIV脑炎有关。采用肝素纯化脑样品，采用免疫印迹法检测HCV抗原，并在星形胶质细胞和巨噬细胞-小胶质细胞中检测HCV免疫反应性。研究结果支持了HCV进入hiv感染的大脑的假设，在那里它可能导致与认知障碍相关的生产性合并感染。
Involvement of the nervous system by human immunodeficiency virus (HIV) continues to be a serious problem. Among individuals with HIV who have a history of illicit drug use, those coinfected with hepatitis C virus (HCV) are a fast- growing population. However, few studies have assessed the penetration of HCV into the central nervous system (CNS) and its clinical and neuropathological impacts on HIV-infected individuals. For this purpose, the distribution of HCV was investigated in the brains of patients infected with HIV. The presence of HCV RNA in the CNS as detected by nested polymerase chain reaction was associated with a history of methamphetamine use, considerable antemortem cognitive impairment and abundant astrogliosis, and lesssevere HIV encephalitis. HCV antigens were detected by immunoblot analysis, using heparin- purified brain samples, and HCV immunoreactivity was detected in astrocytes and in macrophage- microglial cells. The results support the hypothesis that HCV traffics into the HIV-infected brain, where it might lead to a productive coinfection associated with cognitive impairment.