Identification of metabolites of 4,4'-methylenedianiline in vascular smooth muscle cells by liquid chromatography-electrospray tandem mass spectrometry.
Identification of metabolites of 4,4'-methylenedianiline in vascular smooth muscle cells by liquid chromatography-electrospray tandem mass spectrometry.
复制标题
液相色谱-电喷雾串联质谱法鉴定血管平滑肌细胞中 4,4-亚甲基二苯胺的代谢物。
DOI：
10.1002/jms.1026
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Cole,RichardB
中科院分区：
文献类型：
--
作者：
Chen,Kan;Dugas,TammyR;Cole,RichardB
A capillary liquid chromatography‐electrospray tandem mass spectrometry (LC‐ES‐MS/MS) method was developed for the identification of metabolites of 4,4′‐methylenedianiline (diaminodiphenyl methane, (DAPM)) derived from exposure of vascular smooth muscle cells (VSMC) to this compound. The use of precolumn concentration and column switching techniques prior to reversed‐phase liquid chromatography coupled online to ES‐MS enabled the separation and detection of low‐level DAPM and its metabolites in the exposed cell samples. The employed LC‐ES‐MS method, and further LC‐ES‐MS/MS analysis enabled the structural assignments for two DAPM metabolites from vascular smooth muscle cells:N‐acetyl methylenedianiline (N‐acetyl‐DAPM) andN,N′‐diacetyl methylenedianiline (N,N′‐diacetyl‐DAPM). Copyright © 2006 John Wiley & Sons, Ltd.
