Time course analysis of semi-quantitative PCR and antigenaemia assay for prevention of cytomegalovirus disease after bone marrow transplantation
Time course analysis of semi-quantitative PCR and antigenaemia assay for prevention of cytomegalovirus disease after bone marrow transplantation
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DOI：
10.1046/j.1365-2141.1998.00518.x
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发表时间：
1998-01-01
影响因子：
6.5
通讯作者：
Hirai, H
中科院分区：
文献类型：
--
作者：
Kanda, Y;Chiba, S;Hirai, H
巨细胞病毒（CMV）疾病是骨髓移植（BMT）后的严重并发症。最近，开发了 CMV-DNA 的半定量聚合酶链反应 (SQ-PCR) 分析，我们比较了 SQ-PCR 的时程和 BMT 不同的抗原血症测定的时程。我们的研究结果表明，SQ-PCR 在检测 CMV 感染方面比抗原血症测定更敏感，并且 CMV-DNA 定量可以区分 PCR 阳性但没有 CMV 疾病风险的患者。我们认为 SQ-PCR 可以作为针对 CMV 疾病的预防性治疗的有用指南。
Cytomegalovirus (CMV) disease is a serious complication after bone marrow transplantation (BMT). Recently, semi-quantitative polymerase chain reaction (SQ-PCR) analysis for CMV-DNA has been developed, We compared the time course of SQ-PCR and that of antigenaemia assay alter BMT. Our findings suggested that SQ-PCR is more sensitive than antigenaemia assay in the detection of CMV infection and that quantitation of CMV-DNA could discriminate patients with positive PCR but without the risk for CMV disease. We considered that SQ-PCR could be a useful guide for starting pre-emptive therapy against CMV disease.