INVOLVEMENT OF FC-EPSILON-RII/CD23 AND L-ARGININE DEPENDENT PATHWAY IN IGE-MEDIATED ACTIVATION OF HUMAN EOSINOPHILS
INVOLVEMENT OF FC-EPSILON-RII/CD23 AND L-ARGININE DEPENDENT PATHWAY IN IGE-MEDIATED ACTIVATION OF HUMAN EOSINOPHILS
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DOI：
10.1006/bbrc.1994.2177
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发表时间：
1994-08-30
影响因子：
3.1
通讯作者：
MOSSALAYI, MD
中科院分区：
文献类型：
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作者：
AROCK, M;LEGOFF, L;MOSSALAYI, MD
嗜酸性粒细胞显示免疫球蛋白E（IgE）的各种受体，包括IgE的高亲和力受体（Fc ε RI）、CD 23（Fc ε RII）和Mac-2/CD 2BP。我们试图在这里澄清这些受体在IgE介导的激活正常人骨髓培养的嗜酸性粒细胞的作用。用IL-4预处理嗜酸性粒细胞是IgE/抗IgE介导的TNF-α和过氧化物产生刺激所必需的。在CD 23连接后也诱导了嗜酸性粒细胞的TNF-α释放，并且在较小程度上与抗Mac-2连接，而Fc β RI连接没有影响。当用CD 23-m4 b的Fab片段预处理嗜酸性粒细胞时，IgE/抗IgE效应显著降低。此外，N-G-monomethyl-L-arginine抑制CD 23依赖的一氧化氮通路也可逆转这种作用。一氧化氮化学供体SIN-1诱导嗜酸性粒细胞释放TNF-α。因此，CD 23和一氧化氮途径参与IgE介导的正常密度人嗜酸性粒细胞的刺激。(C)1994年出版社出版。
Eosinophils display various receptors for immunoglobulin E (IgE) including the high affinity receptor for IgE (Fc epsilon RI), CD23 (Fc epsilon RII), and Mac-2/epsilon BP. We attempted here to clarify the role of these receptors in IgE-mediated activation of eosinophils from normal human bone marrow cultures. Pretreatment of eosinophils with IL-4 is required for IgE/anti-IgE-mediated stimulation of TNF-alpha and peroxydes production. TNF-alpha release from eosinophils was also induced following ligation of CD23 and to a lesser extent with anti-Mac-2, while Fc epsilon RI-ligation had no effect. IgE/anti-IgE effect dramatically decreased when eosinophils were pretreated with Fab fragments of CD23-m4b. In addition, this effect could also be reversed by inhibiting CD23-dependent nitric oxide pathway by N-G-monomethyl-L-arginine. Nitric oxide chemical donor, SIN-1, induced TNF-a release from eosinophils. CD23 and nitric oxide pathway are thus involved in IgE-mediated stimulation of normodense human eosinophils. (C) 1994 Academic Press, Inc.