Intracellular and extracellular protective mechanisms of the anammox consortia against exogenous sulfadimidine.
Intracellular and extracellular protective mechanisms of the anammox consortia against exogenous sulfadimidine.
复制标题
DOI：
10.1016/j.jhazmat.2022.128817
复制
发表时间：
2022-03
影响因子：
13.6
通讯作者：
Yuan-Long Ma;Zheng-Yang Lu;Jin-Jin Fu-Jin;Nian-Si Fan;R. Jin
中科院分区：
文献类型：
--
作者：
Yuan-Long Ma;Zheng-Yang Lu;Jin-Jin Fu-Jin;Nian-Si Fan;R. Jin
抗生素和抗生素耐药基因（ARGs）已被认为是危及人类和动物健康的新兴高风险污染物。本研究系统研究了畜禽养殖废水中一种典型抗生素（磺胺嘧啶，SDM）对厌氧氨氧化过程的综合影响，旨在阐明厌氧氨氧化菌对抗生素应激的胞内和胞外保护机制。结果表明，高浓度SDM显著降低了厌氧氨氧化活性（SAA），降幅达37.8%。kuenenia candidatuskuenenia丰度的变化趋势与SAA相似，而其他与氮相关的微生物（如NitrosomonasandNitrospira）对氮的去除也有贡献，特别是在抑制期。厌氧氨氧化菌群对SDM的抗性主要依赖于ARGs和EPS的保护。网络分析显示，earg的宿主范围比iarg大，且inti1与代表性反硝化菌关系密切。此外，元蛋白质组学分析和分子对接结果表明，EPS中丰富的蛋白质可以通过氢键形成复合物将SDM滞留在细胞外基质中。研究结果对厌氧氨氧化工艺的稳定运行和ARGs的转移控制具有指导意义。
Antibiotics and antibiotic resistance genes (ARGs) have been recognized as emerging high-risk pollutants for human and animal health. This study systematically investigated the comprehensive effects of a typical antibiotic (sulfadimidine, SDM) in livestock and poultry breeding wastewater on the anammox process, with the aim of elucidating the intracellular and extracellular protective mechanisms of the anammox consortia to the antibiotic stress. Results revealed that the high-concentration SDM significantly reduced the specific anammox activity (SAA) by 37.8%. Changes in the abundance ofCandidatusKuenenia showed a similar trend with that of SAA, while other nitrogen-related microorganisms (e.g.,NitrosomonasandNitrospira) contributed to the nitrogen removal especially during the inhibitory period. Resistance of the anammox consortia to SDM mainly depended on the protection of ARGs and EPS. Network analysis revealed the host range of eARGs was relatively larger than that of iARGs, andintI1 was closely associated with representative denitrifiers. In addition, metaproteomic analysis and molecular docking results indicated that abundant proteins in EPS could detain SDM in the extracellular matrix through forming complex via hydrogen bond. These findings provide a guidance for the stable operation of anammox process and ARGs transfer controlling.