Growth hormone and insulin binding to human liver.
Growth hormone and insulin binding to human liver.
复制标题
生长激素和胰岛素与人肝脏结合。
DOI：
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发表时间：
1976
影响因子：
5.8
通讯作者：
H. G. Friesen
中科院分区：
文献类型：
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作者：
D. Carr;H. G. Friesen
在12 ~ 15例尸检标本中发现125 I-hGH与人肝特异性结合。特异性结合研究使用一种新的技术，采用20亩厚的“微切片”在低温恒温器上切割。每个测定管约0.5至1 mg的组织使得小样本研究可行。特异性结合范围为每mg组织干重加入80，000 cpm 125 I-hGH的1.4 - 11.7%。在肝匀浆和粗膜制备物中也证明了特异性结合。在研究的一系列患者中，未发现125 I-hGH结合与年龄、性别或病理学之间存在相关性。在肺、肾上腺、脾或肾中未观察到125 I-hGH的特异性结合，尽管所有组织均结合125 I-胰岛素。几种生长激素测试，只有灵长类动物的GH取代125 I-hGH从其结合位点在人类肝脏。未观察到羊或人催乳素或胰岛素的置换。灵长类胎盘催乳素在从人肝脏中置换125 I-hGH时仅具有天然hGH的0.5-1.0%效力。然而，有蹄胎盘提取物在这方面与hGH等效。
Specific binding of 125I-hGH to human liver was found in autopsy specimens from 12 to 15 patients. Specific binding was studied using a new technique employing 20 mu thich "microslices" cut on a cryostat. About 0.5 to 1 mg of tissue per assay tube makes feasible the study of small samples. The range of specific binding was 1.4 to 11.7% of 80,000 cpm 125I-hGH added expressed per mg dry weight of tissue. Specific binding was also demonstratable in homogenates and crude membrane preparations from liver. No correlation was seen between 125I-hGH binding and age, sex, or pathology in the series of patients studied. No specific binding of 125I-hGH was observed in lung, adrenal, spleen, or kidney, although all the tissues bound 125I-insulin. Of several species of growth hormone tested, only primate GH displaced 125I-hGH from its binding sites in human liver. No displacement was seen with ovine or human prolactin or with insulin. Primate placental lactogens had only 0.5-1.0% potency of native hGH in displacing 125I-hGH from human liver. Ungulate placental extracts, however, were equipotent with hGH in this respect.