Protection of calves against cryptosporidiosis with immune bovine colostrum induced by a Cryptosporidium parvum recombinant protein
Protection of calves against cryptosporidiosis with immune bovine colostrum induced by a Cryptosporidium parvum recombinant protein
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DOI：
10.1016/s0264-410x(98)00477-0
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发表时间：
1999-04-23
期刊：
影响因子：
5.5
通讯作者：
Hunt, EL
中科院分区：
文献类型：
--
作者：
Perryman, LE;Kapil, SJ;Hunt, EL
本研究的目的是确定用微小隐孢子虫重组蛋白（rC 7）免疫接种是否会诱导免疫牛初乳，保护小牛在用微小隐孢子虫卵囊经口攻击后免受隐孢子虫病。妊娠晚期的荷斯坦奶牛对C.用300 μ g亲和纯化的rC 7 C，parvum重组蛋白免疫parvum三次（免疫奶牛），或保持未免疫（对照奶牛）。从两组中的每头奶牛中获得初乳，并分配至相同的混合免疫初乳或混合对照初乳等分试样中。出生时获得的12头小牛在第一个2小时内接受免疫或对照初乳，并在12和24小时的年龄再次。每头小牛均接受10（7）C经口激发。在12小时龄时对细小卵囊进行接种，并监测隐孢子虫病的体征。给予合并对照初乳的所有6头小牛均发生重度腹泻（平均总粪便量= 8447 +/- 5600 ml），并平均排出1.87 +/- 1.66 x 10（12）个微小C卵囊。给予混合免疫初乳的6头小牛均未出现腹泻（平均总粪便量= 740 +/- 750 ml，p < 0.05），排出的卵囊显著较少（3.05 +/- 2.26 x 10（9），p < 0.05）。免疫牛初乳中未出现腹泻，卵囊排出量减少2.79 log（10）（99.8%），表明用C.小孢子虫重组蛋白rC 7对新生小牛的隐孢子虫病提供了实质性的保护。(C)1999爱思唯尔科技有限公司。保留所有权利。
The purpose of the study was to determine if immunization with a recombinant protein (rC7) of Cryptosporidium parvum would induce immune bovine colostrum that protected calves against cryptosporidiosis following oral challenge with C, parvum oocysts. Late gestation Holstein cows with low titers of antibody to the p23 antigen of C. parvum were immunized three times with 300 mu g affinity purified rC7 C, parvum recombinant protein (immune cows), or left nonimmunized (control cows). Colostrum was obtained from each cow in both groups and partitioned into identical aliquots of pooled immune colostrum or pooled control colostrum. Twelve calves obtained at birth received either immune or control colostrum within the first 2 h, and again at 12 and 24 h of age. Each calf was challenged orally with 10(7) C. parvum oocysts at 12 h of age and monitored for signs of cryptosporidiosis. All six calves administered pooled control colostrum developed severe diarrhea (mean total fecal volume = 8447 +/- 5600 ml) and shed an average of 1.87 +/- 1.66 x 10(12) C, parvum oocysts. None of the six calves administered pooled immune colostrum developed diarrhea (mean total fecal volume = 740 +/- 750 mi, p < 0.05), and shed significantly fewer oocysts (3.05 +/- 2.26 x 10(9), p < 0.05). The absence of diarrhea and 2.79 log(10) (99.8%) reduction in oocyst excretion indicates that immune bovine colostrum induced by immunization with C. parvum recombinant protein rC7 provided substantial protection against cryptosporidiosis in neonatal calves. (C) 1999 Elsevier Science Ltd. All rights reserved.