Towards ultrahigh throughput microinjection: MEMS-based massively-parallelized mechanoporation.
Towards ultrahigh throughput microinjection: MEMS-based massively-parallelized mechanoporation.
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DOI：
10.1109/embc.2012.6346001
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Rao MP
中科院分区：
文献类型：
--
作者：
Zhang Y;Ballas CB;Rao MP
我们描述了一种基于 MEMS 的大规模并行设备概念，用于通过机械膜穿透（即机械穿孔）将外源分子被动输送到活细胞中。讨论了有关设备设计和制造的细节，以及侧重于概念验证级别的设备验证的初步活细胞研究的结果。这些努力代表了我们朝着开发能够通过主动显微注射进行超高通量 (UHT) 细胞操作的仪器的长期目标迈出的关键一步。
We describe a massively-parallelized, MEMS-based device concept for passively delivering exogeneous molecules into living cells via mechanical membrane penetration, i.e., mechanoporation. Details regarding device design and fabrication are discussed, as are results from preliminary live cell studies focused on device validation at the proof-of-concept level. These efforts represent key steps towards our long-term goal of developing instrumentation capable of ultrahigh throughput (UHT) cellular manipulation via active microinjection.