Whispering-gallery-mode biosensing: label-free detection down to single molecules
Whispering-gallery-mode biosensing: label-free detection down to single molecules
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DOI：
10.1038/nmeth.1221
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发表时间：
2008-07-01
期刊：
影响因子：
48
通讯作者：
Arnold, Stephen
中科院分区：
文献类型：
--
作者：
Vollmer, Frank;Arnold, Stephen
无光学标记探测器，例如古老的表面等离子体共振（SPR）传感器，因其能够获取分子间结合的定量数据而普遍受到青睐。然而，在近期引入利用回音壁模式（WGM）再循环的共振微腔之前，对单个结合事件的敏感性尚未实现。在此，我们描述了使WGM生物传感器具有极高灵敏度的增强机制，综述了其当前的实现方式和应用，并讨论了其未来的可能性。
Optical label-free detectors, such as the venerable surface plasmon resonance (SPR) sensor, are generally favored for their ability to obtain quantitative data on intermolecular binding. However, before the recent introduction of resonant microcavities that use whispering gallery mode (WGM) recirculation, sensitivity to single binding events had not materialized. Here we describe the enhancement mechanisms responsible for the extreme sensitivity of the WGM biosensor, review its current implementations and applications, and discuss its future possibilities.