CTGF Loaded Electrospun Dual Porous Core-Shell Membrane For Diabetic Wound Healing
CTGF Loaded Electrospun Dual Porous Core-Shell Membrane For Diabetic Wound Healing
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DOI：
10.2147/ijn.s224047
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发表时间：
2019-01-01
影响因子：
8
通讯作者：
Webster, Thomas J.
中科院分区：
文献类型：
--
作者：
Augustine, Robin;Zahid, Alap Ali;Webster, Thomas J.
目的：伤口愈合受损是 2 型糖尿病的一个主要问题，通常会导致慢性感染，最终导致肢体和/或器官截肢。结缔组织生长因子（CTGF）是一种信号分子，在组织修复和再生中发挥多种作用，包括促进细胞粘附、细胞迁移、细胞增殖和血管生成。将 CTGF 纳入可生物降解的核壳纤维中以促进其持续释放，是促进血管生成、细胞迁移和促进伤口愈合的新方法。在本文中，我们报告了用于糖尿病伤口愈合应用的 CTGF 封装的静电纺丝双多孔 PLA-PVA 核壳纤维膜的开发。方法：该膜是通过核壳静电纺丝技术制造的。 CTGF 被包裹在 PVA 核心内，该核心涂有一层薄薄的 PLA。通过扫描电子显微镜 (SEM)、傅里叶变换红外光谱 (FTIR) 和 X 射线衍射 (XRD) 分析等技术对开发的膜进行了表征。使用成纤维细胞、角质形成细胞和内皮细胞进行体外细胞培养研究，以了解负载 CTGF 的膜对细胞增殖、细胞活力和细胞迁移的影响。进行鸡绒毛尿囊膜（CAM）测定以确定膜的血管生成潜力。结果：结果显示，所开发的膜在形态上具有高度多孔性，并且在单个纤维的表面上形成次生孔。体外细胞培养研究表明，加载 CTGF 的核壳膜可改善细胞活力、细胞增殖和细胞迁移。观察到 CTGF 从核-壳纤维中持续释放较长时间。此外，CAM实验表明，掺入CTGF的核壳膜可以增强血管生成。 结论：由于负载CTGF的核壳PLA-PVA纤维膜具有优异的细胞增殖、迁移和血管生成潜力，它们可以作为一种优良的伤口敷料膜用于治疗糖尿病伤口和其他慢性溃疡。
Purpose: Impairment of wound healing is a major issue in type-2 diabetes that often causes chronic infections, eventually leading to limb and/or organ amputation. Connective tissue growth factor (CTGF) is a signaling molecule with several roles in tissue repair and regeneration including promoting cell adhesion, cell migration, cell proliferation and angiogenesis. Incorporation of CTGF in a biodegradable core-shell fiber to facilitate its sustained release is a novel approach to promote angiogenesis, cell migration and facilitate wound healing. In this paper, we report the development of CTGF encapsulated electrospun dual porous PLA-PVA core-shell fiber based membranes for diabetic wound healing applications.Methods: The membranes were fabricated by a core-shell electrospinning technique. CTGF was entrapped within the PVA core which was coated by a thin layer of PLA. The developed membranes were characterized by techniques such as Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and X-Ray Diffraction (XRD) analysis. In vitro cell culture studies using fibroblasts, keratinocytes and endothelial cells were performed to understand the effect of CTGF loaded membranes on cell proliferation, cell viability and cell migration. A chicken chorioallantoic membrane (CAM) assay was performed to determine the angiogenic potential of the membranes.Results: Results showed that the developed membranes were highly porous in morphology with secondary pore formation on the surface of individual fibers. In vitro cell culture studies demonstrated that CTGF loaded core-shell membranes improved cell viability, cell proliferation and cell migration. A sustained release of CTGF from the core-shell fibers was observed for an extended time period. Moreover, the CAM assay showed that core-shell membranes incorporated with CTGF can enhance angiogenesis.Conclusion: Owing to the excellent cell proliferation, migration and angiogenic potential of CTGF loaded core-shell PLA-PVA fibrous membranes, they can be used as an excellent wound dressing membrane for treating diabetic wounds and other chronic ulcers.