In-chip cell-free protein synthesis from DNA by using biochemical IC chips
In-chip cell-free protein synthesis from DNA by using biochemical IC chips
复制标题
使用生化 IC 芯片从 DNA 进行片内无细胞蛋白质合成
DOI：
10.1109/memsys.2001.906576
复制
发表时间：
2001
期刊：
影响因子：
--
通讯作者：
S. Maruo
中科院分区：
文献类型：
--
作者：
K. Ikuta;A. Takahashi;S. Maruo
在生物化学IC芯片中展示了世界上第一个“基于DNA的芯片内无细胞蛋白质合成”。从DNA中合成了萤火虫荧光素酶的发光蛋白，并对其进行了实时监测。该微型化学实验室由作者提出和开发的预制式生化IC芯片搭建而成。每个芯片都是用“混合微立体光刻术”进行三维制造的。我们的成功结果为后基因组研究寻找基因和蛋白质之间的关系提供了新的强有力的方法。用户组装的多功能生化IC芯片为我们提供了减少实验通过时间，减少昂贵的生化试剂和各种蛋白质仪器的成本。DNA来源的无细胞蛋白质合成不仅是生命科学的最大关键技术之一，而且是医学和药物开发的最大关键技术之一。
World's first "In-chip cell-free protein synthesis from DNA" in the biochemical IC chips was demonstrated. A luminous protein of firefly "Luciferase" was synthesized from DNA and monitored in real time manner. This micro chemical laboratory was constructed by prefabricated biochemical IC chips proposed and developed by the authors. Each chip was three dimensionally fabricated by using "Hybrid micro stereolithography". Our successful results provide new and strong method demanded from post-genome research to find the relationship between gene and protein. User assemble versatile biochemical IC chips provide us to reduces experimental through put time, the cost of expensive biochemical reagent and apparatus for various kinds of protein. DNA originated cell-free protein synthesis is one of the biggest key technology for not only life science but also medicine and drug discovery.
影响因子：
56.9
作者：
SPIRIN, AS;BARANOV, VI;ALAKHOV, YB
通讯作者：
ALAKHOV, YB