Rhizopus oryzae Whole-Cell-Catalyzed Biodiesel Production from Oleic Acid in tert-Butanol Medium†
Rhizopus oryzae Whole-Cell-Catalyzed Biodiesel Production from Oleic Acid in tert-Butanol Medium†
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DOI：
10.1021/ef700624v
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发表时间：
2008
期刊：
影响因子：
5.3
通讯作者：
Wei Li;Wei Du;De‐hua Liu
中科院分区：
文献类型：
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作者：
Wei Li;Wei Du;De‐hua Liu
在利用根霉全细胞代替固定化酶生产生物柴油的过程中，发现其胞内脂肪酶催化甘油三酯甲醇解时具有1，3-位专一性。因此，对R.在甘油三酸酯生物柴油生产过程中，酵母全细胞受到一定程度的抑制。然而，它可能是一个有前途的催化剂，用于从游离脂肪酸（FFA）生产生物柴油。在本文中，R.系统研究了固定化生物质载体颗粒（BSPs）固定化IFO 4697全细胞催化油酸制备生物柴油的性能。在叔丁醇体系中，R。与无溶剂体系相比，无溶剂体系中的全电池具有更好的耐甲醇性和更好的稳定性。在反应混合物中加入分子筛（3A）在线脱除生成水，可获得更高的生物柴油产率（48 h时生物柴油产率达到90%）。
During the usage of Rhizopus oryzae whole cell instead of immobilized enzyme for biodiesel production, the intracellular lipase was found to have 1,3-positional specificity when used to catalyze methanolysis of triglycerides. Thus, the application of R. oryzae whole cell in biodiesel production from triglycerides is restrained to some extent. However, it might be a promising catalyst for biodiesel production from free fatty acids (FFAs). In this paper, R. oryzae IFO4697 whole cell [immobilized within biomass support particles (BSPs)] catalyzed biodiesel production from oleic acid was studied systematically. It has been demonstrated that, in a tert-butanol system, R. oryzae whole cell exhibited both better methanol endurance and better stability than that in a solvent-free system. Molecular sieves (3 A) were added into the reaction mixture to online remove the produced water, and a much higher biodiesel yield could be achieved (biodiesel yield reached 90% at 48 h).