Cell cycle modulation of protein-DNA interactions at a human replication origin
Cell cycle modulation of protein-DNA interactions at a human replication origin
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DOI：
10.1093/emboj/17.10.2961
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发表时间：
1998-05-15
期刊：
影响因子：
11.4
通讯作者：
Falaschi, A
中科院分区：
文献类型：
--
作者：
Abdurashidova, G;Riva, S;Falaschi, A
我们跟踪了在细胞周期的不同时刻同步化的IMR-90细胞中，在位于人核纤层蛋白B2基因附近的早期发射复制起点上体内发生的蛋白质-DNA相互作用的变化。在G（0）期细胞中不存在保护;随着细胞进入G（1）期，出现了覆盖超过100 bp的延伸足迹，特别是在G（1）/S边界处。当细胞进入S期时，保护收缩至70 bp，并且在整个S期内保持不变。在有丝分裂中，这种保护作用完全消失，只有当细胞进入下一个G（1）时，才以伸展的形式重新出现。这些变化让人想起那些对应于酵母和非洲爪蟾细胞中描述的复制前和复制后复合物的形成。
We followed the variations of protein-DNA interactions occurring in vivo over the early firing replication origin located near the human lamin B2 gene, in IMR-90 cells synchronized in different moments of the cell cycle, In G(0) phase cells no protection is present; as the cells progress in G(1) phase an extended footprint covering over 100 bp appears, particularly marked at the G(1)/S border. As the cells enter S phase the protection shrinks to 70 bp and remains unchanged throughout this phase. In mitosis the protection totally disappears, only to reappear in its extended form as the cells move into the next G(1). These variations are reminiscent of those corresponding to the formation of the pre-and post-replicative complexes described in yeast and Xenopus cells.