Effect of selected anti-cataract agents on opacification in the selenite cataract model
Effect of selected anti-cataract agents on opacification in the selenite cataract model
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DOI：
10.1006/exer.1996.0002
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发表时间：
1996-01-01
影响因子：
3.4
通讯作者：
Thurston, GM
中科院分区：
文献类型：
--
作者：
Hiraoka, T;Clark, JI;Thurston, GM
采用亚硒酸盐模型对14种试剂的抗白内障活性进行了系统的研究。这些试剂或它们的衍生物是从文献报道中鉴定出来的，它们对白内障形成的潜在有效性，每个试剂对体外晶状体匀浆的相分离温度T-e的影响被测量，T-e是导致蛋白质聚集的分子相互作用的直接测量。在体内评价了皮下注射每种试剂[1.5 mmol(kg体重)(-1)]对晶状体混浊的保护作用。在体内对晶状体混浊的影响最强，体外对T_1的影响最强。体内作用最弱的是体外对T_1影响最弱的试剂。这一结果暗示了试剂对T的影响与体内防止白内障形成之间的关系。(C)1996年学术出版社有限公司
A systematic study of the anti-cataract activity of 14 reagents was conducted using the selenite model. The reagents or their derivatives were identified from literature reports of their potential effectiveness against cataract formation, The effects of each reagent were measured on the phase separation temperature, T-e, of lens homogenate in vitro, T-e is a direct measure of molecular interactions leading to protein aggregation. The protective effect of a single subcutaneous injection of each reagent [at a dose of 1.5 mmol (kg body weight)(-1)] on lens opacification was evaluated in vivo using rats administered selenite [at a dose of 19 mu mol (kg body weight)(-1)] to initiate cataract formation. The strongest effects on lens opacification in vivo were observed with reagents having the strongest effect on T,, in vitro. The weakest effects in vivo were observed with the reagents having the weakest effect on T,, in vitro. The results were suggestive of a relationship between the effect of a reagent on T, and protection against cataract formation in vivo. (C) 1996 Academic Press Limited