High-throughput analysis of serum antigens using sandwich ELISAs on microarrays.
High-throughput analysis of serum antigens using sandwich ELISAs on microarrays.
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DOI：
10.1007/978-1-60327-811-9_10
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发表时间：
2009
影响因子：
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通讯作者：
S. Servoss;Rachel M. Gonzalez;S. Varnum;R. Zangar
中科院分区：
文献类型：
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作者：
S. Servoss;Rachel M. Gonzalez;S. Varnum;R. Zangar
酶联免疫吸附测定（ELISA）微阵列有望成为检测和验证疾病生物标志物的有力工具。ELISA微阵列能够使用小样本量同时检测许多蛋白质。尽管使用ELISA微阵列存在许多潜在的缺陷，但这些缺陷可以通过仔细规划实验来避免。在本章中，我们描述了处理ELISA微阵列的高通量协议，这将导致可靠和可重复的数据。
Enzyme-linked immunosorbent assay (ELISA) microarrays promise to be a powerful tool for the detection and validation of disease biomarkers. ELISA microarrays are capable of simultaneous detection of many proteins using a small sample volume. Although there are many potential pitfalls to the use of ELISA microarrays, these can be avoided by careful planning of experiments. In this chapter we describe a high-throughput protocol for processing ELISA microarrays that will result in reliable and reproducible data.