Expression and role of RANKL in periodontal ligament cells during physiological root-resorption in human deciduous teeth
Expression and role of RANKL in periodontal ligament cells during physiological root-resorption in human deciduous teeth
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DOI：
10.1034/j.1600-0722.2003.00051.x
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发表时间：
2003-08-01
影响因子：
1.9
通讯作者：
Okabe, K
中科院分区：
文献类型：
--
作者：
Fukushima, H;Kajiya, H;Okabe, K
尽管NF-κB配体受体激活剂（RANKL）及其受体（RANK）在破骨细胞生成和骨吸收中的重要作用已被确定，但它们在生理性牙根吸收过程中的表达和作用仍不确定。人类乳牙脱落的生理性牙根吸收是由破骨细胞样细胞（破牙细胞）介导的。在这项研究中，我们使用免疫细胞化学和逆转录酶聚合酶链反应检测了 RANKL 和骨保护素 (OPG) 的表达，OPG 是一种诱饵受体，可在生理性牙根吸收过程中阻止 RANKL 与人牙周膜 (PDL) 细胞中的 RANK 结合。通过测量磷酸钙涂层盖玻片上溶解面积的大小来评估 RANKL 对破牙细胞根吸收活性的影响。从非吸收乳牙或恒牙分离的 PDL 细胞大量表达 OPG，但不表达 RANKL。相反，源自吸收乳牙的 PDL 细胞主要表达 RANKL。来自吸收乳牙的人类破牙细胞表达 RANKL 和 RANK。据观察，RANKL 以剂量依赖性方式增加破牙细胞肌动蛋白环的形成和再吸收活性。这些结果表明，根吸收状态下的 PDL 细胞表达 RANKL，但降低 OPG 表达。 RANKL 的表达可能参与破牙细胞发生并激活生理性牙根吸收。
Although important roles of receptor activator of NF-kappaB ligand (RANKL) and its receptor (RANK) have been established for osteoclastogenesis and bone resorption, their expression and roles during physiological root resorption remain uncertain. Physiological root resorption for shedding of human deciduous teeth is mediated by osteoclast-like cells (odontoclasts). In this study, we examined the expression of RANKL and osteoprotegerin (OPG), a decoy receptor that prevents RANKL from binding to RANK in human periodontal ligament (PDL) cells during physiological root resorption using immunocytochemistry and reverse transcriptase polymerase chain reaction. The effect of RANKL on root resorbing activity of odontoclasts was evaluated by measuring the size of dissolved area on calcium phosphate-coated coverslips. The PDL cells isolated from either non-resorbing deciduous teeth or permanent teeth abundantly expressed OPG, but not RANKL. In contrast, PDL cells derived from resorbing deciduous teeth dominantly expressed RANKL. Human odontoclasts derived from resorbing deciduous teeth expressed both RANKL and RANK. It was observed that RANKL increased odontoclast actin ring formation and resorbing activity in a dose-dependent manner. These results indicate that PDL cells during the root-resorbing state express RANKL but decrease OPG expression. Expression of RANKL likely participates in odontoclastogenesis and activates physiological root resorption.