ANTIMICROBIAL ACTIVITY AND PHYLOGENETIC ANALYSIS OF STREPTOMYCES PARVULUS DOSMB-D105 ISOLATED FROM THE MANGROVE SEDIMENTS OF ANDAMAN ISLANDS
ANTIMICROBIAL ACTIVITY AND PHYLOGENETIC ANALYSIS OF STREPTOMYCES PARVULUS DOSMB-D105 ISOLATED FROM THE MANGROVE SEDIMENTS OF ANDAMAN ISLANDS
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DOI：
10.1556/030.63.2016.1.2
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发表时间：
2016-03-01
影响因子：
1.5
通讯作者：
Kumar, Ashok
中科院分区：
文献类型：
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作者：
Baskaran, R.;Mohan, P. M.;Kumar, Ashok
放线菌，特别是链霉菌是大量产生药理活性化合物的菌株，约占目前临床使用的天然衍生抗生素的70%。在本研究中，我们用五种溶剂从南安达曼群岛红树林沉积物中分离到海洋微小链霉菌DOSMB-D105的次生代谢产物。其中，乙酸乙酯粗提物对11种病原菌和6种真菌的抑菌活性最强。用GC-MS检测乙酸乙酯提取物中活性物质的存在，并将检测到的化合物与美国国家标准与技术研究所(NIST)文库匹配。共鉴定出8个化合物，其中主要成分为2个甾体、2个生物碱、2个增塑剂、1个酚类和1个烷烃。目前的研究表明，微小链球菌DOSMB-D105是一个很有前途的菌株，可以分离出有价值的生物活性物质来对抗病原微生物。
Actinomycetes, especially species of Streptomyces are prolific producers of pharmacologically significant compounds accounting for about 70% of the naturally derived antibiotics that are presently in clinical use. In this study, we used five solvents to extract the secondary metabolites from marine Streptomyces parvulus DOSMB-D105, which was isolated from the mangrove sediments of the South Andaman Islands. Among them, ethyl acetate crude extract showed maximum activity against 11 pathogenic bacteria and six fungi. Presence of bioactive compounds in the ethyl acetate extract was determined using GC-MS and the compounds detected in the ethyl acetate extract were matched with the National Institute of Standards and Technology (NIST) library. Totally eight compounds were identified and the prevalent compounds were 2 steroids, 2 alkaloids, 2 plasticizers, 1 phenolic and 1 alkane. Present study revealed that S. parvulus DOSMB-D105 is a promising species for the isolation of valuable bioactive compounds to combat pathogenic microbes.