Reactivated biofilm coupling n-DAMO with anammox achieved high-rate nitrogen removal in membrane aerated moving bed biofilm reactor
Reactivated biofilm coupling n-DAMO with anammox achieved high-rate nitrogen removal in membrane aerated moving bed biofilm reactor
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n-DAMO与厌氧氨氧化偶联的再活化生物膜在膜曝气移动床生物膜反应器中实现高效脱氮
DOI：
10.1016/j.envres.2022.115184
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发表时间：
2023
影响因子：
8.3
通讯作者：
Guo-Jun Xie
中科院分区：
文献类型：
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作者：
Zhi-Cheng Zhao;Sheng-Qiang Fan;Yang Lu;Cheng-Cheng Dang;Xuan Wang;Bing-Feng Liu;De-Feng Xing;Jun Ma;Nan-Qi Ren;Qilin Wang;Guo-Jun Xie
依赖硝酸盐/亚硝酸盐的厌氧甲烷氧化(n-达摩)与厌氧氨氧化相结合是一种很有前途的技术，为实现高效、可持续的废水处理提供了一种解决方案。然而，这一可持续的过程面临着积累足够的生物量以达到实际脱氮性能的挑战。本研究开发了一种新型的膜曝气移动床生物膜反应器，该反应器支持充足的甲烷供应和良好的生物膜附着性，用于培养n-达摩和厌氧氨氧化耦合的生物膜。在氨水和硝酸盐的作用下，生物膜在聚氨酯泡沫塑料上迅速生长，在102d内达到了275g/Nm−3D−1的生物反应器性能。在−20℃保存8个月后，生物膜被成功地重新激活，188天后达到315g/N m−3D−1。经复活后，采用MAMBBR工艺处理合成侧流废水。在4.0kg.Nm−3D−1的生物反应器性能下，脱氮率达到99.9%。微生物群落分析和质量平衡计算表明，n-达摩微生物和厌氧氨氧化细菌共同促进了MAMBBR的脱氮。本研究开发的MAMBBR为污水的可持续处理提供了一种将n-达摩与厌氧氨氧化相结合的理想系统。
As a promising technology, the combination of nitrate/nitrite-dependent anaerobic methane oxidation (n-DAMO) with Anammox offers a solution to achieve effective and sustainable wastewater treatment. However, this sustainable process faces challenges to accumulate sufficient biomass for reaching practical nitrogen removal performance. This study developed an innovative membrane aerated moving bed biofilm reactor (MAMBBR), which supported sufficient methane supply and excellent biofilm attachment, for cultivating biofilms coupling n-DAMO with Anammox. Biofilms were developed rapidly on the polyurethane foam with the supply of ammonium and nitrate, achieving the bioreactor performance of 275 g N m−3d−1within 102 days. After the preservation at −20 °C for 8 months, the biofilm was successfully reactivated and achieved 315 g N m−3d−1after 188 days. After reactivation, MAMBBR was applied to treat synthetic sidestream wastewater. Up to 99.9% of total nitrogen was removed with the bioreactor performance of 4.0 kg N m−3d−1. Microbial community analysis and mass balance calculation demonstrated that n-DAMO microorganisms and Anammox bacteria collectively contributed to nitrogen removal in MAMBBR. The MAMBBR developed in this study provides an ideal system of integrating n-DAMO with Anammox for sustainable wastewater treatment.