THE C-CBL PROTO-ONCOGENE IS PREFERENTIALLY EXPRESSED IN THYMUS AND TESTIS TISSUE AND ENCODES A NUCLEAR-PROTEIN
THE C-CBL PROTO-ONCOGENE IS PREFERENTIALLY EXPRESSED IN THYMUS AND TESTIS TISSUE AND ENCODES A NUCLEAR-PROTEIN
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DOI：
10.1128/jvi.63.12.5420-5424.1989
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发表时间：
1989-12-01
影响因子：
5.4
通讯作者：
MORSE, HC
中科院分区：
文献类型：
--
作者：
LANGDON, WY;HYLAND, CD;MORSE, HC
CAS NS-1是一种急性转化的小鼠逆转录病毒，可导致早期B系淋巴瘤和偶尔的髓系白血病。这种病毒的转化序列v-cbl与已知的癌基因没有同源性，但与酵母转录因子Gcn4有一些相似之处。在这项研究中，我们使用v-CBL探针分析了广泛的小鼠和人类造血肿瘤细胞系的mRNAs，并在所有谱系中检测到11kb的mRNAs。在正常小鼠组织中，c-CBL在睾丸和胸腺组织中的表达最高，在睾丸组织中的优势物种是3.5kb的mRNA。将v-cbl癌基因插入到细菌表达载体中，获得兔免疫蛋白。亲和纯化的v-CBL抗体在Cas NS-1转化的成纤维细胞中检测到大量的p100gag-CBL，在正常和转化的细胞中都检测到低水平的135kodalton蛋白(p135c-CBL)。亚细胞分离表明，p100gag-cbl和p135c-cbl均位于细胞核内，提取420 mM盐后，p135c-cbl仍保留在细胞核内。这些结果表明，c-CBL的翻译产物是一个135千道尔顿的核蛋白。
Cas NS-1 is an acutely transforming murine retrovirus that induces early B-lineage lymphomas and occasional myeloid leukemias. The transforming sequence of this virus, v-cbl, shows no homology to known oncogenes but has some similarities to the yeast transcriptional factor GCN4. In this study we used a v-cbl probe to analyze mRNAs from a wide range of murine and human hemopoietic tumor cell lines and detected an 11-kilobase mRNA in all lineages. In normal mouse tissues the expression of c-cbl was highest in testis and thymus tissues, the predominant species in testis tissue being a 3.5-kilobase mRNA. The v-cbl oncogene was inserted into a bacterial expression vector to produce protein for the immunization of rabbits. Affinity-purified v-cbl antibodies identified abundant levels of p100gag-cbl in Cas NS-1-transformed fibroblasts and lower levels of a 135-kilodalton protein (p135c--cbl) in both normal and transformed cells. Subcellular fractionation showed that p100gag-cbl and p135c-cbl are both located in the nucleus and retained following 420 mM salt extraction. These results indicate that the translational product of a c-cbl is a 135-kilodalton nuclear protein.