General anesthesia delays the inflammatory response and increases survival for mice with endotoxic shock
General anesthesia delays the inflammatory response and increases survival for mice with endotoxic shock
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DOI：
10.1128/cvi.13.2.281-288.2006
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发表时间：
2006-02-01
影响因子：
--
通讯作者：
De Maio, A
中科院分区：
文献类型：
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作者：
Fuentes, JM;Talamini, MA;De Maio, A
麻醉是任何手术过程中不可缺少的组成部分。在这项研究中，我们证明了在致死剂量（20 mg/kg体重）大肠杆菌脂多糖（LPS）后持续异氟醚麻醉1小时，与未麻醉的小鼠相比，C57BL/6J（136）小鼠的存活率显著提高。麻醉保护与血浆LPS循环延迟相关，导致炎症反应延迟，特别是nf - κ B的DNA结合活性和肿瘤坏死因子α、白细胞介素6 （IL-6）和IL-10的血清水平。不同种类的麻醉剂对炎症反应产生相同的效果，这也与使用的近亲繁殖小鼠品系无关。这些结果提示麻醉对内毒素血症的预后有重要影响。此外，在解释实验动物模型的数据时，应考虑麻醉剂的免疫调节作用。
Anesthesia is an indispensable component of any operative procedure. In this study, we demonstrate that continuous isoflurane anesthesia for 1 h after a lethal dose (20 mg/kg of body weight) of Escherichia coli lipopolysaccharide (LPS) results in a significant increase in survival of C57BL/6J (136) mice in comparison with survival of nonanesthetized mice. Protection by anesthesia correlates with a delay in plasma LPS circulation, resulting in a delayed inflammatory response, particularly DNA binding activity of NF-kappa B and serum levels of tumor necrosis factor alpha, interleukin-6 (IL-6), and IL-10. Disparate classes of anesthetic agents produce the same effects on the inflammatory response, which is also independent of the inbred mouse strain used. These results suggest that anesthesia has an important impact on the outcome from endotoxemia. Moreover, the immunomodulatory effects of anesthetics should be considered when interpreting data from experimental animal models.