An ovine model of postinfarction dilated cardiomyopathy
An ovine model of postinfarction dilated cardiomyopathy
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DOI：
10.1016/s0003-4975(02)03827-4
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发表时间：
2002-09-01
影响因子：
4.6
通讯作者：
Gorman, RC
中科院分区：
文献类型：
--
作者：
Moainie, SL;Gorman, JH;Gorman, RC
背景冠状动脉病变是导致充血性心力衰竭的主要原因，但目前尚无合适的心肌梗死后扩张型心肌病动物模型。本文描述了一个绵羊模型，发展后的前基底梗死。用亚甲蓝和四氮唑注射法分别测定了20只屠宰羊和8只活羊左肠系膜动脉前两对角支心肌的分布。另外7只动物出现梗死，并接受了超过8周的系列血流动力学、微球和超声心动图研究，并在第八周进行了组织学研究。在屠宰场和活的心脏中，心肌重量分别占左心室重量的24.6% ± 4.7%和23.9% ± 2.2%。在重构过程中，左心室收缩末期和舒张末期容积分别增加115%和73%，射血分数从41.2% +/- 6.7%降至29.1% +/-5.7%，远离梗死的收缩期室壁增厚减少68%，球形指数从0.465 ± 0.088增加到0.524 ± 0.038，左心室舒张末期压从1.7 ± 1.0增加到8.2 ± 3.5 mm Hg。连续微球测量记录了所有未梗死心肌的正常血流量（1.34 mL/g/min）和22%的梗死正常血流量。存活心肌可见轻度间质纤维化。该绵羊模型符合梗死后扩张型心肌病的所有标准，并且具有控制冠状动脉解剖结构、侧支血管分布的变化以及混淆该疾病发病机制的人类研究的动脉粥样硬化病变的数量、位置和严重程度的差异的优点。这个简单的模型仅包含由中等大小的局部梗死产生的梗死和充分灌注的低收缩心肌。(C)2002年由胸外科医师协会出版。
Background. Coronary arterial disease is the major cause of congestive heart failure, but suitable animal models of postinfarction, dilated cardiomyopathy do not exist. This article describes an ovine model that develops after an anterobasal infarction.Methods. The distribution of ovine myocardium supplied by the first two diagonal branches of the left homonymous artery were determined in 20 slaughterhouse hearts and eight live sheep using methylene blue and tetrazolium injections, respectively. Seven additional animals had the infarction and underwent serial hemodynamic, microsphere and echocardiographic studies more than 8 weeks and histologic studies at the eighth week. Infarcts represented 24.6% +/- 4.7% and 23.9% +/- 2.2% of the left ventricular mass in slaughterhouse and live hearts, respectively.Results. During remodeling, left ventricular endsystolic and end-diastolic volumes increased 115% and 73%, respectively, ejection fraction decreased from 41.2% +/- 6.7% to 29.1% +/- 5.7%, systolic wall thickening remote from the infarct decreased by 68%, sphericity index increased from 0.465 +/- 0.088 to 0.524 +/- 0.038, and left ventricular end-diastolic pressure increased from 1.7 +/- 1.0 to 8.2 +/- 3.5 mm Hg. Serial microsphere measurements documented normal blood flow (1.34 mL/g per minute) to all uninfarcted myocardium and 22% of normal to the infarct. Viable myocardium showed mild interstitial fibrosis.Conclusions. This ovine model meets all criteria for postinfarction, dilated cardiomyopathy and has the advantages of controlling for variations in coronary arterial anatomy, collateral vascularity, and differences in the numbers, location, and severity of atherosclerotic lesions that confound human studies of the pathogenesis of this disease. This simple model contains only infarcted and fully perfused, hypocontractile myocardium produced by a moderate-sized, regional infarction.(C) 2002 by The Society of Thoracic Surgeons.