Differential usage of non-homologous end-joining and homologous recombination in double strand break repair
Differential usage of non-homologous end-joining and homologous recombination in double strand break repair
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DOI：
10.1016/j.dnarep.2006.05.022
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发表时间：
2006-09-08
期刊：
影响因子：
3.8
通讯作者：
Takeda, Shunichi
中科院分区：
文献类型：
--
作者：
Sonoda, Eiichiro;Hochegger, Helfrid;Takeda, Shunichi
DNA双链断裂（DSB）的修复在基因组的维持中起着关键作用。DSB的出现经常是由于复制叉停滞以及外源因子的攻击。修复受损的DNA对生存至关重要。两个主要途径，同源重组（HR）和非同源末端连接（NHEJ）已经进化到处理这些病变，并且从酵母到脊椎动物都是保守的。尽管这些途径的保护，它们对DSB修复的相对贡献在这两个物种之间差异很大。HR在酵母中的任何DSB修复中起主导作用，而NHEJ在脊椎动物中显着有助于DSB修复。这种活跃的NHEJ需要一种调节机制来选择脊椎动物细胞中的HR或NHEJ。在这篇综述中，我们说明了HR和NHEJ是如何根据细胞周期的阶段和DSB的性质差异调节。(c)2006 Elsevier B.V.保留所有权利。
Repair of DNA double strand breaks (DSBs) plays a critical role in the maintenance of the genome. DSB arise frequently as a consequence of replication fork stalling and also due to the attack of exogenous agents. Repair of broken DNA is essential for survival. Two major pathways, homologous recombination (HR) and non-homologous end-joining (NHEJ) have evolved to deal with these lesions, and are conserved from yeast to vertebrates. Despite the conservation of these pathways, their relative contribution to DSB repair varies greatly between these two species. HR plays a dominant role in any DSB repair in yeast, whereas NHEJ significantly contributes to DSB repair in vertebrates. This active NHEJ requires a regulatory mechanism to choose HR or NHEJ in vertebrate cells. In this review, we illustrate how HR and NHEJ are differentially regulated depending on the phase of cell cycle and on the nature of the DSB. (c) 2006 Elsevier B.V. All rights reserved.