Evidence for many resolvable structures within conformation types of electrosprayed ubiquitin ions
Evidence for many resolvable structures within conformation types of electrosprayed ubiquitin ions
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DOI：
10.1021/jp056165h
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发表时间：
2006-04-06
影响因子：
3.3
通讯作者：
Clemmer, DE
中科院分区：
文献类型：
--
作者：
Koeniger, SL;Merenbloom, SI;Clemmer, DE
一种新的二维离子淌度光谱法与质谱法相结合已用于检查气相中的泛素离子。在这种方法中，离子在初始漂移管中被分离成构象类型（由它们的碰撞横截面定义），然后使用门将窄分布的迁移率分离的离子引入第二个漂移管中以进行后续分离。结果表明，选择后，通过第二个漂移管保留了窄峰形状。这要求在 300 K 时，所选分布在与这些实验类似的 10-20 ms 时间范围内与构象类型相关的更广泛的结构范围内基本上不会相互转换。对于 [M + 7H](7+) 离子，似乎可以在先前定义的每种紧凑、部分折叠和拉长构象异构体类型中进行许多（类似于 5-10）窄选择。
A new two-dimensional ion mobility spectrometry approach combined with mass spectrometry has been used to examine ubiquitin ions in the gas phase. In this approach ions are separated in an initial drift tube into conformation types (defined by their collision cross sections) and then a gate is used to introduce a narrow distribution of mobility-separated ions into a second drift tube for subsequent separation. The results show that upon selection a narrow peak shape is retained through the second drift tube. This requires that at 300 K the selected distribution does not interconvert Substantially within the broader range of structures associated with the conformation type within the similar to 10-20 ms time scale of these experiments. For the [M + 7H](7+) ion, it appears that many (similar to 5-10) narrow selections can be made across each of the compact, partially-folded, and elongated conformer types, defined previously.