Development of a comprehensive analytical method for furanocoumarins in grapefruit and their metabolites in plasma and urine using UPLC-MS/MS: a preliminary study
Development of a comprehensive analytical method for furanocoumarins in grapefruit and their metabolites in plasma and urine using UPLC-MS/MS: a preliminary study
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DOI：
10.1080/09637486.2016.1207157
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发表时间：
2016-01-01
影响因子：
3.9
通讯作者：
Chun, Ock K.
中科院分区：
文献类型：
--
作者：
Lee, Sang Gil;Kim, Kijoon;Chun, Ock K.
为了开发光活性呋喃香豆素的综合分析方法，使用 QuEChERS（快速、简单、廉价、有效、耐用和安全）方法提取葡萄柚（整个、果肉、果皮和果汁）。使用 UPLC-MS/MS 测定了葡萄柚中的七种呋喃香豆素：香柠檬醇、补骨脂素、8-甲氧基补骨脂素、香柠檬烯、6,7-二羟基香柠檬素 (6,7-DHB)、环氧香柠檬素和香柠檬素。还测定了六名健康年轻人在摄入葡萄柚或葡萄柚汁之前和之后血浆和尿液中呋喃香豆素的浓度。通过 QuEChERS 方法从基质加标样品和实验室校准样品中呋喃香豆素的回收率分别为 125.7 +/- 25.4% 和 105.7 +/- 6.3%。佛手柑和 6,7-DHB 是葡萄柚果肉、果汁和血浆中的主要化合物，而佛手柑醇和 6,7-DHB 是尿液中检测到的主要化合物。结果表明佛手柑和6,7-DHB被代谢为佛手柑醇。总体而言，本研究开发的分析方法可应用于植物来源和生物样品中各种呋喃香豆素的分析。
To develop a comprehensive analytical method for photoactive furanocoumarins, grapefruit (whole, flesh, peel and juice) was extracted using QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method. Seven furanocoumarins: bergaptol, psoralen, 8-methoxypsoralen, bergapten, 6,7-dihydroxybergamottin (6,7-DHB), epoxybergamottin and bergamottin were determined in grapefruit using UPLC-MS/MS. The concentrations of furanocoumarins in the plasma and urine of six healthy young adults before and after ingestion of grapefruit or grapefruit juice were also determined. Recovery rates of furanocoumarins by QuEChERS method from matrix spike sample and laboratory calibrate sample were 125.7 +/- 25.4% and 105.7 +/- 6.3%, respectively. Bergamottin and 6,7-DHB were predominant compounds in grapefruit flesh, juice and plasma, while bergaptol and 6,7-DHB were major compounds detected in the urine. The results demonstrated that bergamottin and 6,7-DHB were metabolized to bergaptol. Overall, the analytical methods developed in the present study can be applied to the analysis of various furanocoumarins in plant sources and biological samples.