Intracellular synthesis of d-aminoluciferin for bioluminescence generation.
Intracellular synthesis of d-aminoluciferin for bioluminescence generation.
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DOI：
10.1039/c7cc00999b
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发表时间：
2017-03
影响因子：
4.9
通讯作者：
Zhen Zheng;Gongyu Li;Chengfan Wu;Miaomiao Zhang;Yue Zhao;G. Liang
中科院分区：
文献类型：
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作者：
Zhen Zheng;Gongyu Li;Chengfan Wu;Miaomiao Zhang;Yue Zhao;G. Liang
d-荧光素是生物发光（BL）应用中最广泛使用的底物，但其低化学稳定性总是影响其性能。在此，我们合理地设计了两种化学稳定的前体分子CBT-d-胱氨酸-CBT（d-1）和CBT-l-胱氨酸-CBT（l-1），并使它们进行还原控制缩合以形成1-寡聚体，随后进行蛋白水解以产生d-氨基荧光素用于在细胞和体内产生BL。我们设想，我们的前体分子可能会在不久的将来作为一个广泛的BL应用的替代品。
d-Luciferin is the most widely used substrate for bioluminescence (BL) applications but its low chemical stability always affects its performance. Herein, we rationally designed two chemically stable precursor molecules CBT-d-cystine-CBT (d-1) and CBT-l-cystine-CBT (l-1), and subjected them to reduction-controlled condensation to form 1-oligomer and subsequent proteolysis to yield d-aminoluciferin for BL generation in cells and in vivo. We envision that our precursor molecules might serve as d-luciferin alternatives for a wide range of BL applications in the near future.