Proteome analysis of Streptomyces coelicolor mutants affected in the proteasome system reveals changes in stress-responsive proteins
Proteome analysis of Streptomyces coelicolor mutants affected in the proteasome system reveals changes in stress-responsive proteins
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DOI：
10.1007/s00203-007-0243-8
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发表时间：
2007-09-01
影响因子：
2.8
通讯作者：
Nagy, Istvan
中科院分区：
文献类型：
--
作者：
De Mot, Rene;Schoofs, Geert;Nagy, Istvan
原核生物的20S蛋白酶体仅限于古细菌和放线菌。这种区隔化的多亚基蛋白酶的细菌靶点尚未确定，其在原核生物中的生理功能仍然未知。在本研究中，我们分析了冷色链霉菌A3(2)突变体的胞内和胞外蛋白质组，包括20S蛋白酶体结构基因、推定的蛋白酶体辅助AAA atp酶ARC编码基因，以及两个推定的蛋白酶体相关放线菌特异性基因（sco1646、sco1647），揭示了应激反应蛋白的改变模式。此外，sco1647突变体的胞外蛋白酶谱也发生了显著改变。四种突变体最显著的变化是非血红素氯过氧化物酶SCO0465水平的显著增加，与对异丙苯过氧化氢的抗性增加相一致。
Prokaryotic 20S proteasomes are confined to archaebacteria and actinomycetes. Bacterial targets of this compartmentalized multi-subunit protease have not yet been identified and its physiological function in prokaryotes remains unknown. In this study, intracellular and extracellular proteomes of Streptomyces coelicolor A3(2) mutants affected in the structural genes of the 20S proteasome, in the gene encoding the presumed proteasome-accessory AAA ATPase ARC, or in two putative proteasome-associated actinomycete-specific genes (sco1646, sco1647) were analysed, revealing modified patterns of stress-responsive proteins. In addition, the extracellular protease profile of the sco1647 mutant was significantly altered. The most prominent change, common to the four mutants, was a strongly increased level of the non-heme chloroperoxidase SCO0465, coinciding with an increased resistance to cumene hydroperoxide.