Injection of 1,25-(OH)2 vitamin D3 enhances resistance artery contractile properties.
Injection of 1,25-(OH)2 vitamin D3 enhances resistance artery contractile properties.
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注射 1,25-(OH)2 维生素 D3 可增强阻力动脉收缩特性。
DOI：
10.1161/01.hyp.16.5.523
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Wagman,DW
中科院分区：
文献类型：
--
作者：
Bukoski,RD;Wang,DB;Wagman,DW
检验了1，25-二羟维生素D3 [1，25-（OH）2维生素D3]调节血管平滑肌收缩功能的假设。13-15周龄雄性自发性高血压大鼠和Wistar-Kyoto血压正常大鼠在3天内通过腹膜内注射给予1，25-（OH）2维生素D3（50 ng/天）。第四天，制备血清，并检查离体肠系膜阻力动脉的收缩力产生。用1，25-（OH）2维生素D3治疗大约使激素的血清水平加倍，并使离子化和总血清Ca 2+和磷酸盐增加5- 10%。未检测到对血压的影响。在两种品系中注射1，25-（OH）2维生素D3使去甲肾上腺素和5-羟色胺的最大应激产生增强30- 40%，对血管对激动剂的表观敏感性没有影响。为了评估在不添加外源性激素的情况下升高血清离子化Ca 2+的策略的效果，在从喂食含有0.5%或2%钙的饮食的大鼠中分离的阻力动脉中检查最大应力产生，持续6-7周。最大应力生成反应去甲肾上腺素是更大的血管从大鼠的两个菌株保持在0.5%的钙。结论是，用1，25-（OH）2维生素D3体内治疗72小时可增加阻力动脉的收缩力产生能力，而不影响血压。有人提出，1，25-（OH）2维生素D3的这种作用是激素对血管壁直接作用的结果。
The hypothesis that 1,25-dihydroxyvitamin D3 [1,25-(OH)2 vitamin D3] modulates vascular smooth muscle contractile function was tested. 1,25-(OH)2 vitamin D3 (50 ng/day) was administered by intraperitoneal injection over a 3-day period to 13-15-week-old male spontaneously hypertensive and Wistar-Kyoto normotensive rats. On the fourth day, serum was prepared and contractile force generation of isolated mesenteric resistance arteries was examined. Treatment with 1,25-(OH)2 vitamin D3 approximately doubled serum levels of the hormone and increased ionized and total serum Ca2+ and phosphate by 5-10%. No effect on blood pressure was detected. 1,25-(OH)2 vitamin D3 injection in both strains enhanced maximal stress generation to norepinephrine and serotonin by 30-40%, with no effect on apparent sensitivity of the vessels to the agonists. To assess the effect of a maneuver that elevates serum ionized Ca2+ without the addition of exogenous hormone, maximal stress generation was examined in resistance arteries isolated from rats fed diets containing 0.5% or 2% calcium over a 6-7-week period. Maximal stress generation in response to norepinephrine was greater in vessels from rats of both strains maintained on 0.5% calcium. It is concluded that 72-hour in vivo treatment with 1,25-(OH)2 vitamin D3 increases contractile force-generating capacity of resistance arteries without affecting blood pressure. It is proposed that this action of 1,25-(OH)2 vitamin D3 is the result of a direct action of the hormone on the vascular wall.