Highly sensitive solution-gated graphene transistors for label-free DNA detection
Highly sensitive solution-gated graphene transistors for label-free DNA detection
复制标题
用于无标记 DNA 检测的高灵敏度溶液门控石墨烯晶体管
DOI：
10.1016/j.bios.2019.04.034
复制
发表时间：
2019-07-01
影响因子：
12.6
通讯作者：
Li, Jinhua
中科院分区：
文献类型：
--
作者：
Li, Shanshan;Huang, Kang;Li, Jinhua
由于DNA分子在检测多种疾病的诊断和治疗中的遗传损伤和错误方面的重要作用，人们非常希望进行高灵敏度和低成本的DNA检测。传统的DNA检测主要依靠大型仪器，检测过程复杂，成本高。溶液栅极电化学晶体管因其灵敏度高、成本低而得到广泛的研究。在这里，我们开发了一种石墨烯电化学晶体管，用于高效和灵敏的DNA检测。将探针DNA修饰在门电极上，以检测目标DNA分子。提出了一种用探针DNA修饰栅电极来检测不同浓度的靶DNA的新方法。因此，与以前报道的方法相比，我们的DNA传感器在1 fM-5微米的范围内表现出良好的检测极限。高灵敏度和选择性验证了所开发的DNA传感器是一种有希望的常规使用工具。
Highly sensitive and low-cost DNA detection are in great desire owing to the significant role of DNA molecules in detecting genetic damage and errors for the diagnosis and treatment of multiple diseases. Traditional detections of DNA mainly rely on large-scale instrument, which requires the complicated detection process and high cost. Solution-gated electrochemical transistors are widely studied due to high sensitivity and low cost. Here, we exploit a graphene electrochemical transistor for the efficient and sensitive DNA detection. The probe DNA is modified on the gate electrode to detect the target DNA molecules. A novel method is introduced to modify the gate electrode with the probe DNA to detect the different concentration of target DNA. Herein, in comparison with previous reported methods, our DNA sensors show a good limit of detection in the range of 1 fM - 5 mu M. The highly sensitive and selective validate the developed DNA sensor as a promising tool for routine use.