Sindbis virus infection of two model insect cell systems - A comparative study
Sindbis virus infection of two model insect cell systems - A comparative study
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DOI：
10.1016/j.virusres.2006.06.007
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发表时间：
2006-12-01
期刊：
影响因子：
5
通讯作者：
Brown, Dennis T.
中科院分区：
文献类型：
--
作者：
Mudiganti, Usharani;Hernandez, Raquel;Brown, Dennis T.
甲病毒的原型Sindbis在哺乳动物中引起蚊子传播的疾病，并在各种脊椎动物和无脊椎动物细胞培养物中复制。这一特点可以利用大量的果蝇遗传信息来研究Sindbis病毒与另一种无脊椎动物宿主的相互作用。为此，对施奈德-2型果蝇（S2）细胞与白纹伊蚊（克隆U4.4）细胞的Sindbis病毒感染情况进行了比较研究。感染后，脊椎动物细胞在24-48小时内死亡，而无脊椎动物细胞培养物在感染的急性期存活并持续感染。在本研究中，比较了模型果蝇系统S2细胞与U4.4细胞的感染情况。与U4.4细胞相比，研究了S2细胞感染后的病毒产生、持久性建立的时间过程和生长特性的变化。S2细胞在急性Sindbis感染后存活，没有任何明显的细胞病理变化，并继续产生低水平的病毒，这是持续感染细胞的特征。S2细胞感染后第1天产生10个PFU/细胞，第2天下降到2个PFU/细胞。这一结果与U4.4细胞相反，U4.4细胞在感染后第2天产生峰值病毒滴度，并在第5天建立持久性。U4.4或S2细胞感染持续期的开始没有导致任何形态或生长特征的改变。本研究建立了S2细胞作为一个额外的无脊椎动物模型系统来研究无脊椎动物宿主与Sindbis病毒的相互作用。(c) 2006 Elsevier B.V.版权所有
Sindbis, the prototype of the Alphaviruses causes mosquito-borne diseases in mammals and replicates in a wide variety of vertebrate and invertebrate cell cultures. This characteristic can be exploited to use the vast array of Drosophila genetic information available for investigations of the interaction of Sindbis virus with an alternate invertebrate host. For this purpose, a comparative study of Sindbis virus infection of Schnieder-2 Drosophila (S2) cells to cells of the mosquito Aedes albopictus (clone U4.4) was undertaken. After infection, vertebrate cells die within 24-48 h, while invertebrate cell cultures survive an acute phase of infection and become persistently infected. In this study, infection of a model Drosophila system, S2 cells, was compared to U4.4 cells. Virus production, the time course of the establishment of persistence and changes in growth properties of the S2 cells upon infection, were studied in comparison to those of the U4.4 cells. S2 cells survived acute Sindbis infection without any significant cytopathology and continued to produce low levels of virus characteristic of persistently infected cells. S2 cells produced 10 PFU/cell on day 1 post-infection, which falls to 2 PFU/cell on day 2. This result is in contrast to U4.4 cells, which produce peak virus titer on day 2 post-infection and establish persistence by day 5. Onset of the persistent phase of infection of either U4.4 or S2 cells did not result in any change in morphology or growth characteristics. This study establishes S2 cells as an additional invertebrate model system to study the interactions of an invertebrate host with Sindbis virus. (c) 2006 Elsevier B.V. All rights reserved.