Characterization of inhibitory constituents of NO production from Catalpa ovata using LC-MS coupled with a cell-based assay
Characterization of inhibitory constituents of NO production from Catalpa ovata using LC-MS coupled with a cell-based assay
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DOI：
10.1016/j.bioorg.2018.05.023
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发表时间：
2018-10-01
影响因子：
5.1
通讯作者：
Lee, Ki Yong
中科院分区：
文献类型：
--
作者：
Park, Sangmin;Shin, Hyeji;Lee, Ki Yong
提出了一种利用LC-QTOF MS/MS结合细胞分析筛选天然产物中NO生成抑制剂的有效方法。梓乙酸乙酯部分表现出较强的抑制作用，在脂多糖诱导的BV2小胶质细胞NO的产生。我们试图鉴定C.通过使用LC-QTOF MS/MS结合基于细胞的测定法对卵形体进行分析。MS色谱图上约14 - 15 min处的峰估计为生物活性成分。从白藜芦醇乙酸乙酯部位分离得到一个新环烯醚萜类化合物6-O-反式阿魏酰-3 β-羟基-7-脱氧rehamaglutin A（4）和9个已知化合物（1 - 3，5 - 10）。反复柱层析分离纯化。化合物3、4、5、7和8显著减弱了BV2细胞中脂多糖刺激的NO产生。我们的研究结果表明，LC-QTOF MS/MS结合基于细胞的NO产生抑制试验成功地预测了活性化合物，而无需耗时的分离过程。
An effective screening method for inhibitors of NO production in natural products using LC-QTOF MS/MS coupled with a cell-based assay was proposed. The ethyl acetate fraction of Catalpa ovata exhibited a strong inhibitory effect on NO production in lipopolysaccharide-induced BV2 microglia cells. We attempted to identify the active constituents of C. ovata by using LC-QTOF MS/MS coupled with a cell-based assay. Peaks at approximately 14-15 min on the MS chromatogram were estimated to be the bioactive constituents. A new iridoid compound, 6-O-trans-feruloyl-3 beta-hydroxy-7-deoxyrehamaglutin A (4), and nine known compounds (1-3, 5-10) were isolated from the ethyl acetate fraction of C. ovata by repeated column chromatography. Compounds 3, 4, 5, 7, and 8 significantly attenuated lipopolysaccharide-stimulated NO production in BV2 cells. Our results indicate that LC-QTOF MS/MS coupled with a cell-based NO production inhibitory assay successfully predicted active compounds without a time-consuming isolation process.