Estimation of total carbohydrate amount in environmental samples by the phenol-sulphuric acid method assisted by multivariate calibration
Estimation of total carbohydrate amount in environmental samples by the phenol-sulphuric acid method assisted by multivariate calibration
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DOI：
10.1016/j.chemolab.2005.04.005
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发表时间：
2005-10-28
影响因子：
3.9
通讯作者：
Mecozzi, M
中科院分区：
文献类型：
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作者：
Mecozzi, M
本文对苯酚-硫酸比色法测定环境样品中总碳水化合物的方法进行了改进。由于在环境样品中可以存在不同类型的碳水化合物，因此常规单变量校准中用作标准样品的葡萄糖溶液被同时包含中性、醛酸和氨基糖的溶液所取代，以更好地模拟真实样品的定性组成。然后，主成分回归（PCR）或偏最小二乘（PLS）取代单变量校准。PCR和PLS比单变量校准更准确，因为它们能够考虑到光谱吸收的非加性贡献，这取决于同时存在于中性碳水化合物、醛酸和氨基糖的溶液中。此外，该方法在中性和醛酸亚组分的形成中具有一定的应用潜力。(c) 2005 Elsevier B.V.版权所有
In this paper a modification of the well known phenol-sulphuric acid method for the colorimetric determination of total carbohydrates in environmental samples is proposed. As in environmental samples different typologies of carbohydrates can be present, the solutions of glucose used as standard samples for conventional univariate calibration are replaced by solutions including neutral, uronic acid and aminosugars simultaneously to simulate better the qualitative composition of real samples. Then, principal component regression (PCR) or partial least squares (PLS) replace the univariate calibration. PCR and PLS show to be more accurate than univariate calibration because they are able to take into account the non-additive contributions to the spectral absorptions depending on the simultaneous presence in solution of neutral carbohydrates, uronic acids and aminosugars. In addition, the proposed procedure shows to have some potential applications in the speciation of neutral and uronic acid sub-fractions. (c) 2005 Elsevier B.V. All rights reserved.