MICRODIALYSIS OF LIPOPHILIC COMPOUNDS - A METHODOLOGICAL STUDY
MICRODIALYSIS OF LIPOPHILIC COMPOUNDS - A METHODOLOGICAL STUDY
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DOI：
10.1111/j.1600-0773.1991.tb01315.x
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发表时间：
1991-11-01
期刊：
影响因子：
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通讯作者：
STAHLE, L
中科院分区：
文献类型：
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作者：
CARNEHEIM, C;STAHLE, L
研究了灌注介质对C-14-油酸酯微透析探针体外回收率的影响。 研究了四种灌注介质：水、4%牛血清白蛋白（BSA）的林格溶液、2.25%甘油的水溶液和20%大豆油/卵磷脂乳剂（Intradroid（R））。 发现以下回收顺序（首先是最高回收率）：BSA = Intralidid（R）>甘油>水。 在透析探头灌流速率为0.5 μ l/min时，回收率分别为4.8%、4.4%、2.6%和1.2%。 尝试在静脉输注后测量C-14-油酸酯。 用己烷/异丙醇浸提样品，以将游离脂肪酸与亲水性氧化产物分离。 将透析探针植入大鼠肩胛间脂肪组织、附睾脂肪、肌肉、肝脏和颈静脉。 我们无法检测到未结合的脂肪酸，无论是在基础上还是在用1 mg/kg去甲肾上腺素腹腔内刺激后。 然而，输注油酸盐的氧化产物在去甲肾上腺素刺激下释放。 可以得出结论，微透析装置中的亲脂性化合物的回收率可以通过亲脂性灌注介质或通过例如化合物结合的BSA来改善。 在本研究中，游离脂肪酸水平太小，无法在体内测量。
The influence of perfusion medium on in vitro recovery of C-14-oleate to microdialysis probes was investigated. Four perfusion media were investigated: water, 4% bovine serum albumine (BSA) in Ringer solution, 2.25% glycerol in water and a 20% soybeanoil/eggphospholipid emulsion (Intralipid(R)). The following order of recovery was found (highest recovery first): BSA = Intralipid(R) > glycerol > water. The recoveries at 0.5-mu-l/min. perfusion rate of the dialysis probe were 4.8%, 4.4%, 2.6% and 1.2% respectively. It was attempted to measure C-14-oleate after intravenous infusion. The samples were extracted with hexane/isopropylalcohol in order to separate the free fatty acid from products of oxidation which are hydrophilic. Dialysis probes were implanted in interscapular adipose tissue, epididymal fat, muscle, liver and jugular vein of rats. We were not able to detect unbound fatty acids, neither basally nor after stimulation with 1 mg/kg norepinephrine intraperitoneally. However, products of oxidation from the infused oleate were released in response to norepinephrine stimulation. It is concluded that the recovery of lipophilic compounds in microdialysis devices can be improved by means of a lipophilic perfusion medium or by means of e.g. BSA to which the compound binds. The free fatty acid levels were too small to be measureable in vivo in the present study.