A Demonstration of Cat Seminal Plasma Antifertility Activity
A Demonstration of Cat Seminal Plasma Antifertility Activity
复制标题
猫精浆抗生育活性的演示
DOI：
10.3181/00379727-145-37757
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发表时间：
1974
影响因子：
--
通讯作者：
C. Hamner
中科院分区：
文献类型：
--
作者：
Keith C. McLaughlin;C. Hamner
CSP对家兔精子具有可逆的、部分热不稳定的抗生育作用。在获能的体内受精试验中，CSP处理的精子使卵子受精的数量显著低于对照精子（P < 0.005）。将CSP加热到60° 10分钟会破坏部分活性，而将其加热到100° 10分钟则会进一步减弱其活性。在这两种情况下，CSP处理的精子仍然比对照精子使更少的卵子受精（P = 0.005）。CSP还含有部分热不稳定的胰蛋白酶抑制剂活性，这可能是抗生育活性的部分原因。这项工作的部分支持由赠款HD 06573和职业发展奖12,760我们之一（C。E. H.）从USPHS。
Summary CSP has a reversible, partially heat-labile, antifertility effect on rabbit spermatozoa. In an in vivo fertilization assay for capacitation, CSP-treated sperm fertilize significantly fewer ova than do control sperm (P < 0.005). Heating the CSP to 60° for 10 min destroys some of the activity, while heating it to 100° for 10 min further attenuates it. In both cases, CSP-treated sperm still fertilize fewer ova than do control sperm (P = 0.005). CSP also contains partially heat-labile trypsin inhibitor activity which may be partially responsible for the antifertility activity. This work supported in part by Grant HD06573 and Career Development Award 12,760 to one of us (C. E. H.) from the USPHS.