Flow Cytometric Isolation and Differentiation of Adipogenic Progenitor Cells into Brown and Brite/Beige Adipocytes.
Flow Cytometric Isolation and Differentiation of Adipogenic Progenitor Cells into Brown and Brite/Beige Adipocytes.
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DOI：
10.1007/978-1-4939-6820-6_4
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发表时间：
2017
影响因子：
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通讯作者：
J. Steinbring;A. Graja;Anne-Marie Jank;T. Schulz
中科院分区：
文献类型：
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作者：
J. Steinbring;A. Graja;Anne-Marie Jank;T. Schulz
除了成熟的脂肪细胞，脂肪组织还含有几种不同的细胞群，包括免疫细胞、内皮细胞和成脂祖细胞（AdPC）。AdPC代表了再生细胞的储存库，其在正常细胞周转期间和在对脂质储存能力的需求增加期间使脂肪细胞再生。与代谢综合征相关的病理学（例如肥胖症和2型糖尿病）在世界范围内的增加提高了公众和科学对脂肪组织以及脂肪组织形成和功能的细胞生物学过程的兴趣。已知两种不同类型的脂肪细胞：白色和棕色脂肪细胞。特别是棕色脂肪组织（BAT）由于其独特的产热能量消耗能力和在肥胖症治疗中的潜在作用而受到相当大的关注。因此，冷诱导的白色脂肪细胞向棕色样脂肪细胞的转化已成为人类的可行方法和啮齿动物的研究主题，以更好地理解潜在的分子过程。流式细胞术（FACS）提供了一种方法，通过使用抗体检测独特的表面标志物，从脂肪组织中分离AdPC和其他细胞群。在这里，我们描述了一种方法来分离细胞致力于成脂谱系和总结建立协议，在体外条件下分化FACS纯化的原代腺病毒成UCP1表达棕色脂肪细胞。
Aside from mature adipocytes, adipose tissue harbors several distinct cell populations including immune cells, endothelial cells, and adipogenic progenitor cells (AdPCs). AdPCs represent the reservoir of regenerative cells that replenishes adipocytes during normal cellular turnover and during times of increased demand for triglyceride-storage capacity. The worldwide increase in pathologies associated with the metabolic syndrome, such as obesity and type-2 diabetes, has heightened public and scientific interest in adipose tissues and the cell biological processes of adipose tissue formation and function. Two distinct types of fat cells are known: White and brown adipocytes. Especially brown adipose tissue (BAT) has received considerable attention due to its unique capacity for thermogenic energy expenditure and potential role in the treatment of adiposity. Accordingly, the cold-induced conversion of white into brown-like adipocytes has become a feasible approach in humans and a study-subject in rodents to better understand the underlying molecular processes. Fluorescence-activated cell sorting (FACS) provides a method to isolate AdPCs and other cell populations from adipose tissue by using antibodies detecting unique surface markers. We here describe an approach to isolate cells committed to the adipogenic lineage and summarize established protocols to differentiate FACS-purified primary AdPCs into UCP1-expressing brown adipocytes under in vitro conditions.