Effects of Virus Dose and Extrinsic Incubation Temperature on Vector Competence of Culex nigripalpus (Diptera: Culicidae) for St. Louis Encephalitis Virus
Effects of Virus Dose and Extrinsic Incubation Temperature on Vector Competence of Culex nigripalpus (Diptera: Culicidae) for St. Louis Encephalitis Virus
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DOI：
10.1603/me12054
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发表时间：
2012-11-01
影响因子：
2.1
通讯作者：
Tabachnick, Walter J.
中科院分区：
文献类型：
--
作者：
Richards, Stephanie L.;Anderson, Sheri L.;Tabachnick, Walter J.
黑须库蚊西奥博尔德（Culex nigripalpus Theobald）是美国东南部圣路易斯脑炎病毒的主要媒介。库蚊给黑须鼠雌性喂食含有低（4.0 +/-0.01 log（10）噬斑形成单位当量（PFUeq）/ml）或高（4.7 +/-0.1 log（10）PFUeq/ml）圣路易斯脑炎病毒剂量的血液，并在25或28 ℃的外源孵育温度（EIT）下保持12天。通过定量实时逆转录聚合酶链反应测定载体能力，以使用感染率、传播率和传播率估计PFUeq。在任一剂量下，两种EIT之间的感染率无差异。低剂量组在两个EIT期的感染率均较高。在两种剂量下，28 ℃下的传播率显著高于25 ℃。病毒传播的观察（
Culex nigripalpus Theobald is a primary vector of St. Louis encephalitis virus in the southeastern United States. Cx. nigripalpus females were fed blood containing a low (4.0 +/- 0.01 log(10) plaque-forming unit equivalents (PFUeq)/ml) or high (4.7 +/- 0.1 log(10) PFUeq/ml) St. Louis encephalitis virus dose and maintained at extrinsic incubation temperatures (EIT) of 25 or 28 degrees C for 12 d. Vector competence was measured via quantitative real-time reverse transcriptase polymerase chain reaction to estimate PFUeq using rates of infection, dissemination, and transmission. There were no differences in infection rates between the two EITs at either dose. The low dose had higher infection rates at both EITs. Dissemination rates were significantly higher at 28 degrees C compared with 25 degrees C at both doses. Virus transmission was observed (