Production of reactive oxygen species in peripheral blood is increased in individuals with Helicobacter pylori infection and decreased after its eradication
Production of reactive oxygen species in peripheral blood is increased in individuals with Helicobacter pylori infection and decreased after its eradication
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DOI：
10.1111/j.1523-5378.2006.00410.x
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发表时间：
2006-08-01
期刊：
影响因子：
4.4
通讯作者：
Inoue, Masayasu
中科院分区：
文献类型：
--
作者：
Mashimo, Masayuki;Nishikawa, Manabu;Inoue, Masayasu
背景：据报道幽门螺杆菌感染可导致胃十二指肠溃疡、粘膜相关淋巴组织淋巴瘤和胃癌。最近的研究表明，幽门螺杆菌感染还可能通过未知机制与其他疾病相关，包括血液学和皮肤病以及心血管损伤。 方法：使用高灵敏检测仪测定 86 名患者（34 名幽门螺杆菌阴性受试者和 52 名幽门螺杆菌阳性受试者）的外周血样本中活性氧 (ROS) 的产生。 化学发光探针，L-012（8-氨基-5-氯-7-苯基吡啶并（3,4-d）哒嗪-1和4（2H，3H）二酮）。还分析了 11 名幽门螺杆菌阳性个体在根除幽门螺杆菌后外周血中 ROS 的产生情况。结果：幽门螺杆菌感染个体的 ROS 产生量显着高于未感染个体。根除幽门螺杆菌后，ROS 的增强产生显着下降。 ROS 产生程度与性别、年龄、吸烟状况、饮酒、药物使用或血清 C 反应蛋白水平之间没有发现相关性。结论：这些发现表明，幽门螺杆菌感染导致外周血中 ROS 产生增强。使用 L-012 对血液样本进行化学发光分析可以评估幽门螺杆菌感染患者的全身氧化应激。
Background: Helicobacter pylori infection has been reported to cause gastroduodenal ulcer, mucosa-associated lymphoid tissue lymphoma, and gastric cancer. Recent studies have suggested that H. pylori infection may also associate with other diseases, including hematologic and dermatologic disorders, and cardiovascular injury, by unknown mechanisms.Methods: Production of reactive oxygen species (ROS) was determined in peripheral blood samples from 86 patients (34 H. pylori-negative and 52 H. pylori-positive subjects) using a highly sensitive chemiluminescence probe, L-012 (8-amino-5-chloro-7-phenylpyrido(3,4-d) pyridazine-1 and 4 (2H, 3H) dione). Eleven H. pylori-positive individuals were also analyzed their ROS production in peripheral blood after H. pylori eradication.Results: ROS production was significantly higher in individuals with H. pylori infection than in those without infection. Enhanced production of ROS was decreased significantly after eradication of H. pylori. No correlation was found between the extent of ROS production and sex, age, smoking status, alcohol ingestion, use of medications, or serum level of C-reactive protein.Conclusions: These findings suggest that ROS production was enhanced in peripheral blood by H. pylori infection. Chemiluminescence analysis of blood samples using L-012 permits evaluation of systemic oxidative stress in patients with H. pylori infection.