Expression of CDC5L is associated with tumor progression in gliomas
Expression of CDC5L is associated with tumor progression in gliomas
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CDC5L 的表达与神经胶质瘤的肿瘤进展相关
DOI：
10.1007/s13277-015-4088-5
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发表时间：
2016-03-01
期刊：
影响因子：
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通讯作者：
Wang, Yuchan
中科院分区：
文献类型：
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作者：
Chen, Wenjuan;Zhang, Li;Wang, Yuchan
细胞分裂周期5样蛋白（CDC 5L）是细胞周期G2/M转换的调节因子，参与前信使RNA（mRNA）剪接和DNA损伤修复的催化步骤。最近，它也被发现作为一个候选癌基因在骨肉瘤和宫颈肿瘤。然而，CDC 5L表达在肿瘤生物学中的作用仍不清楚。本文分析了CDC 5L在胶质瘤中的表达及其临床意义。分别采用免疫印迹法和免疫组化法检测CDC 5L在新鲜胶质瘤组织和石蜡包埋脑片中的表达。我们发现CDC 5L在胶质瘤组织中高表达。CDC 5L的表达与胶质瘤的病理分级和Ki-67的表达显著相关。单因素和多因素分析显示，CDC 5L高表达是影响胶质瘤患者生存的独立预后因素。为探讨CDC 5L是否能调节胶质瘤细胞的增殖，我们将靶向CDC 5L的干扰RNA转染胶质瘤细胞，用CCK-8细胞计数试剂盒、流式细胞仪和集落形成分析检测细胞增殖。我们的研究结果表明，CDC 5L基因的敲低可以抑制胶质瘤细胞的增殖。此外，CDC 5L表达降低可诱导胶质瘤细胞凋亡。这些结果提示CDC 5L在胶质瘤中可能发挥重要作用，有望成为胶质瘤治疗的新靶点。
Cell division cycle 5-like (CDC5L) protein is a cell cycle regulator of the G2/M transition and has been reported to participate in the catalytic step of pre-messenger RNA (mRNA) splicing and DNA damage repair. Recently, it was also found to act as a candidate oncogene in osteosarcoma and cervical tumors. However, the role of CDC5L expression in tumor biology was still unclear. Here, we analyzed the expression and clinical significance of CDC5L in gliomas. The expression of CDC5L in fresh glioma tissues and paraffin-embedded slices was evaluated by western blot and immunohistochemistry, respectively. We found that CDC5L was highly expressed in glioma tissues. The expression of CDC5L was significantly associated with glioma pathology grade and Ki-67 expression. Univariate and multivariate analyses showed that high CDC5L expression was an independent prognostic factor for glioma patients’ survival. To determine whether CDC5L could regulate the proliferation of glioma cells, we transfected glioma cells with interfering RNA target CDC5L, then investigated cell proliferation with cell counting kit (CCK)-8, flow cytometry assays and colony formation analyses. Our results indicated that knockdown of CDC5L would inhibit proliferation of glioma cells. Besides, reduced expression of CDC5L could induce the apoptosis of glioma cells. These findings suggested that CDC5L might play an important role in glioma and thus be a promising therapeutic target of glioma.