Loss of keratin 13 in oral carcinoma in situ: a comparative study of protein and gene expression levels using paraffin sections
Loss of keratin 13 in oral carcinoma in situ: a comparative study of protein and gene expression levels using paraffin sections
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口腔原位癌中角蛋白 13 的丢失：使用石蜡切片进行蛋白质和基因表达水平的比较研究
DOI：
10.1111/j.1365-2559.2010.03732.x
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Saku T
中科院分区：
文献类型：
--
作者：
Ida-Yonemochi H;Maruyama S;Kobayashi T;Yamazaki M;Cheng J;Saku T
角蛋白(K)13的免疫组织化学丢失是鉴别口腔黏膜原位癌和非恶性肿瘤的最有价值的诊断指标之一，而K13稳定地定位于正常口腔黏膜的棘细胞。为了阐明K13缺失的分子机制，我们用原位杂交的方法比较了K13和K16在正常舌黏膜上皮中不表达，而在CIS中表达增强的免疫组织化学图谱，并与23例手术切除的舌癌组织经福尔马林固定的石蜡切片中K13和K16的mRNA水平进行了比较。其中7例采用激光显微切割连续石蜡切片上皮细胞的RNA样本进行逆转录聚合酶链式反应。虽然在这7例患者中，K16在蛋白和基因水平均有更高的表达水平，但在4例患者中，K13的缺失与被抑制的mRNA水平有关，而在另外3例患者中，K13基因的缺失与被抑制的mRNA水平无关。结果表明，K13基因的缺失部分是由于它的基因抑制，但也可能是由于一些未知的翻译后事件。
Immunohistochemical loss of keratin (K) 13 is one of the most valuable diagnostic criteria for discriminating carcinoma in situ (CIS) from non-malignancies in the oral mucosa while K13 is stably immunolocalized in the prickle cells of normal oral epithelium. To elucidate the molecular mechanism for the loss of K13, we compared the immunohistochemical profiles for K13 and K16 which is not expressed in normal epithelia, but instead enhanced in CIS, with their mRNA levels by in-situ hybridization in formalin-fixed paraffin sections prepared from 23 CIS cases of the tongue, which were surgically removed. Reverse transcriptase-PCR was also performed using RNA samples extracted from laser-microdissected epithelial fragments of the serial paraffin sections in seven of the cases. Although more enhanced expression levels for K16 were confirmed at both the protein and gene levels in CIS in these seven cases, the loss of K13 was associated with repressed mRNA levels in four cases, but not in the other three cases. The results suggest that the loss of K13 is partly due to its gene repression, but may also be due to some unknown post-translational events.