Rapeseed mitochondrial ccb206, a gene involved in cytochrome c biogenesis, is co-transcribed with the nad3 and rps12 genes: organization, transcription, and RNA editing of the nad3/rps12/ccb206 locus
Rapeseed mitochondrial ccb206, a gene involved in cytochrome c biogenesis, is co-transcribed with the nad3 and rps12 genes: organization, transcription, and RNA editing of the nad3/rps12/ccb206 locus
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油菜线粒体 ccb206 是一种参与细胞色素 c 生物发生的基因，与 nad3 和 rps12 基因共转录：nad3/rps12/ccb206 基因座的组织、转录和 RNA 编辑
DOI：
10.1007/s002940050402
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发表时间：
1998
期刊：
影响因子：
2.5
通讯作者：
H. Handa
中科院分区：
文献类型：
--
作者：
K. Itani;H. Handa
本文描述了油菜（Brassica napusL.）线粒体基因组中thenad 3/rps 12/ccb 206位点的结构和表达。相关物种。在油菜线粒体基因组中，存在三个串联的开放阅读框，分别是NADH脱氢酶亚基3（nad 3）、线粒体核糖体S12亚基蛋白（rps 12）和ABC血红素转运蛋白亚基的同源物（ccb 206）。油菜线粒体中的nad 3/rps 12/ccb 206位点的基因结构在拟南芥线粒体中并不保守，cb 206和nad 3/rps 12位点是相互分离的。我们在这里表明，油菜的基因组织模式也存在于其他油菜品种，但没有在萝卜，一个物种非常密切相关的芸苔属。北方杂交和RT-PCR分析表明这三个线粒体基因可能是共转录的。在油菜nad 3、rps 12和ccb 206转录本中分别发生了10、7和39个C到U RNA编辑事件。最后，我们发现nad 3的密码子位置21在相关的油菜种（包括油菜籽和萝卜）中以高度种特异性的方式被编辑。
We describe here the structure and expression of thenad3/rps12/ccb206locus in the mitochondrial genomes of rapeseed (Brassica napusL.) and related species. In the rapeseed mitochondrial genome there are three tandemly located open reading frames for subunit 3 of NADH dehydrogenase (nad3), the S12 subunit protein of the mitochondrial ribosome (rps12), and a homologue of one subunit of the ABC-heme transporter (ccb206). The gene organization of thenad3/rps12/ccb206locus found in rapeseed mitochondria is not conserved inArabidopsis thalianamitochondria, where theccb206andnad3/rps12loci are separated from each other. We show here that the rapeseed gene-organization pattern is also present in otherBrassicaspecies, but not in radish, a species very closely related toBrassica. Northern hybridization and RT-PCR analyses indicate that these three mitochondrial genes are possibly co-transcribed. Ten, seven, and 39 C-to-U RNA editing events occur in the rapeseednad3,rps12, andccb206transcripts, respectively. Finally we show that codon position 21 ofnad3is edited in a highly species-specific manner among relatedBrassicaspecies, including rapeseed and radish.