Molecular typing of Treponema pallidum strains from patients with neurosyphilis in Pretoria, South Africa
Molecular typing of Treponema pallidum strains from patients with neurosyphilis in Pretoria, South Africa
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DOI：
10.1136/sti.2006.023895
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发表时间：
2007-06-01
影响因子：
3.6
通讯作者：
Hoosen, A. A.
中科院分区：
文献类型：
--
作者：
Molepo, J.;Pillay, A.;Hoosen, A. A.
目的：应用南非比勒陀利亚地区神经梅毒患者脑脊液标本对梅毒螺旋体进行分子分型。方法：采集疑似晚期神经梅毒患者的脑脊液标本，男32例，女18例。T pallidum的分型包括PCR扩增和tpr E、G和J基因的限制性分析，并通过PCR扩增确定arp基因内60个碱基对串联重复序列的个数。结果：在13份可分型标本中，鉴定出4种菌株类型：2i、3e、14a和17e。14a亚型7例（53.8%），3e亚型4例（30.7%），17e和2i亚型各1例（7.6%）。结论：本研究表明，该分型系统可用于可能含有少量螺旋体的标本，如脑脊液。
Objective: To evaluate the molecular typing system for Treponema pallidum using cerebrospinal fluid ( CSF) specimens obtained from patients with neurosyphilis in Pretoria, South Africa.Methods: CSF specimens were collected from 32 men and 18 women with suspected late neurosyphilis. Typing of T pallidum involved PCR amplification and restriction analysis of the tpr E, G and J genes and determination of the number of 60 base pair tandem repeats within the arp gene by PCR amplification.Results: Of 13 typeable specimens, 4 strain types were identified: 2i, 3e, 14a and 17e. Subtype 14a was identified in 7 specimens ( 53.8%), subtype 3e in 4 specimens ( 30.7%) and subtypes 17e and 2i in 1 specimen ( 7.6%) each.Conclusions: This study shows that the typing system can be applied to specimens which may contain low numbers of spirochaetes such as CSF.