Pomegranate phenolics from the peels, arils, and flowers are antiatherogenic:: Studies in vivo in atherosclerotic apolipoprotein E-deficient (Eo) mice and in vitro in cultured macrophages and upoproteins
Pomegranate phenolics from the peels, arils, and flowers are antiatherogenic:: Studies in vivo in atherosclerotic apolipoprotein E-deficient (Eo) mice and in vitro in cultured macrophages and upoproteins
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DOI：
10.1021/jf071811q
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发表时间：
2008-02-13
影响因子：
6.1
通讯作者：
Rosenblat, Mira
中科院分区：
文献类型：
--
作者：
Aviram, Michael;Volkova, Nina;Rosenblat, Mira
我们分析了体内和体外的。与全果汁（PJ）相比，所有石榴果实部分：果皮（POMA, POMxp），假皮（POMA），种子（POMo）和花（POMf）的抗动脉粥样硬化特性和作用机制。动脉粥样硬化Eo小鼠连续3个月服用POM提取物[200 μ g没食子酸当量< GAE)/只/天]。然后采集血液样本、腹腔巨噬细胞（MPM）和主动脉。所有POM提取物均具有体外抗氧化特性。E-o小鼠服用PJ、POMA、POMxp、POMA或POW后，与安慰剂治疗组相比，动脉粥样硬化病变面积分别显著减少了44%、38%、39%、6%或70%，而POMo没有影响。食用POMf可使血脂和葡萄糖水平降低18-25%。与安慰剂治疗的小鼠相比，PJ、POMA、POMxp、POW或POMA的摄入导致MPM总过氧化物含量显著下降，分别下降了53,42,35,27或13%，并增加了细胞对氧磷酶2 （PON2）活性。食用PJ、POMA或POMxp后，E-o-MPM对氧化ldl的摄取率显著降低了15%。在J774A.1巨噬细胞株上也得到了类似的结果。最后，石榴酚类物质（石榴苷、石榴苷、没食子酸和鞣花酸）以及石榴独特的络合糖可以模拟石榴提取物的抗动脉粥样硬化作用。我们的结论是，某些POM提取物，特别是POW，可能与对血脂水平和巨噬细胞致动脉粥样硬化特性的综合有益作用有关。
We have analyzed in vivo and in vitro the. antiatherogenic properties and mechanisms of action of all pomegranate fruit parts: peels (POMA, POMxp), arils (POMa), seeds (POMo), and flowers (POMf), in comparison to whole fruit juice (PJ). Atherosclerotic Eo mice consumed POM extracts [200 mu g of gallic acid equivalents < GAE)/mouse/day] for 3 months. Blood samples, peritoneal macrophages (MPM), and aortas were then collected. All POM extracts possess antioxidative properties in vitro. After consumption of PJ, POMA, POMxp, POMa, or POW by E-o mice, the atherosclerotic lesion area was significantly decreased by 44, 38, 39, 6, or 70%, respectively, as compared to placebo-treated group, while POMo had no effect. POMf consumption reduced serum lipids, and glucose levels by 18-25%. PJ, POMA, POMxp, POW, or POMa consumption resulted in a significant decrement, by 53, 42, 35, 27, or 13%, respectively, in MPM total peroxides content, and increased cellular paraoxonase 2 (PON2) activity, as compared to placebo-treated mice. The uptake rates of oxidized-LDL by E-o-MPM were significantly reduced by similar to 15% after consumption of PJ, POMA, or POMxp. Similar results were obtained on using J774A.1 macrophage cell line. Finally, pomegranate phenolics (punicalagin, punicalin, gallic acid, and ellagic acid), as well as pomegranate unique complexed sugars, could mimic the antiatherogenic effects of pomegranate extracts. We conclude that attenuation of atherosclerosis development by some of the POM extracts and, in particular, POW, could be related to the combined beneficial effects on serum lipids levels and on macrophage atherogenic properties.