ANALYSIS OF NEUROGENESIS IN A MAMMALIAN NEUROEPITHELIUM - PROLIFERATION AND DIFFERENTIATION OF AN OLFACTORY NEURON PRECURSOR INVITRO
ANALYSIS OF NEUROGENESIS IN A MAMMALIAN NEUROEPITHELIUM - PROLIFERATION AND DIFFERENTIATION OF AN OLFACTORY NEURON PRECURSOR INVITRO
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DOI：
10.1016/0896-6273(89)90120-7
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发表时间：
1989-07-01
期刊：
影响因子：
16.2
通讯作者：
CHIKARAISHI, DM
中科院分区：
文献类型：
--
作者：
CALOF, AL;CHIKARAISHI, DM
哺乳动物嗅觉上皮培养系统的发展使神经发生的过程得以在体外进行检测。抗体标记允许明确地识别假定的神经上皮干细胞(角蛋白+基底细胞)和分化的神经元(N-CAM+嗅觉感受器神经元)。结合[~3H]胸腺嘧啶核苷摄取分析，这些抗体被用来表征该系统中直接神经元前体的存在、增殖和分化。这种细胞不同于基底层细胞，它能迅速从基底层细胞中分离出来，在迁移过程中分裂。提供的数据表明，前体遵循一个简单的谱系程序，分裂产生两个N-CAM+子神经元。虽然这种前体在特定的介质中有效地产生神经元，但神经发生随后停止，因为没有产生新的前体，这表明表观遗传因素可能调节该系统中持续的神经发生。
Development of a culture system for mammalian olfactory epithelium has permitted the process of neurogenesis to be examined in vitro. Antibody markers allowing the unambiguous identification of putative neuroepithelial stem cells (keratin+basal cells) and differentiated neurons (N-CAM+olfactory receptor neurons) are described. In combination with [3H]thymidine uptake analysis, these antibodies have been used to characterize the existence, proliferation, and differentiation of the immediate neuronal precursor in this system. This cell is distinct from basal cells and rapidly sorts out from them, dividing as it migrates. Data are presented which suggest that the precursor follows a simple lineage program, dividing to give rise to two N-CAM+daughter neurons. Although this precursor efficiently generates neurons in defined medium, neurogenesis subsequently ceases because new precursors are not produced, suggesting that epigenetic factors may regulate continual neurogenesis in this system.