Automated, high-throughput assays for evaluation of human pancreatic islet function
Automated, high-throughput assays for evaluation of human pancreatic islet function
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DOI：
10.3727/000000007783472408
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发表时间：
2007-01-01
影响因子：
3.3
通讯作者：
Caicedo, Alejandro
中科院分区：
文献类型：
--
作者：
Cabrera, Over;Jacques-Silva, M. Caroline;Caicedo, Alejandro
与I型糖尿病相关的胰岛移植中的一个重要挑战是定义用于评估人类胰岛功能的自动高通量测定。目前使用的生理学技术适用于小规模的实验样品，并产生描述性的结果。后基因组时代为更大规模地分析生物过程提供了机会，但向高通量技术的过渡仍然是一个挑战。作为实施高通量检测来研究人类胰岛功能的第一步，我们开发了两种方法：多次自动灌流来确定胰岛激素分泌，以及高通量动力学成像来检查胰岛细胞反应。这两种技术都使用全自动设备，允许对多个胰岛制剂进行同时实验。我们的研究结果表明，这些技术可以应用于研究功能状态和探索胰岛细胞的药理学概况。这些方法将使移植前的人胰岛制剂的功能特性，从而提供了基础，建立预测测试β细胞的效力。
An important challenge in pancreatic islet transplantation in association with type I diabetes is to define automatic high-throughput assays for evaluation of human islet function. The physiological techniques presently used are amenable to small-scale experimental samples and produce descriptive results. The postgenomic era provides an opportunity to analyze biological processes on a larger scale, but the transition to high-throughput technologies is still a challenge. As a first step to implement high-throughput assays for the study of human islet function, we have developed two methodologies: multiple automated perifusion to determine islet hormone secretion and high-throughput kinetic imaging to examine islet cellular responses. Both technologies use fully automated devices that allow performing simultaneous experiments on multiple islet preparations. Our results illustrate that these technologies can be applied to study the functional status and explore the pharmacological profiles of islet cells. These methodologies will enable functional characterization of human islet preparations before transplantation and thereby provide the basis for the establishment of predictive tests for beta-cell potency.