Production of Cell‐Free Xanthan Fermentation Broth by Cell Adsorption on Fibers
Production of Cell‐Free Xanthan Fermentation Broth by Cell Adsorption on Fibers
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DOI：
10.1021/bp9700770
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发表时间：
1998
影响因子：
2.9
通讯作者：
Shangtian Yang;Yang-Ming Lo;Devamita Chattopadhyay
中科院分区：
文献类型：
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作者：
Shangtian Yang;Yang-Ming Lo;Devamita Chattopadhyay
黄原胶是一种广泛用于食品和石油钻探工业的微生物多糖。目前工业发酵生产的黄原胶中含有大量的细胞和细胞碎片，降低了黄原胶溶液的过滤性能，限制了其应用。因此，无细胞黄原胶发酵液的生产是合乎需要的。研究了不同编织纤维材料对黄原胶发酵液中菌体的吸附去除效果。结果表明，棉纤维和聚酯纤维均可用于吸附发酵液中的黄单胞菌细胞，无论是在发酵过程中还是发酵后。几乎所有的细胞都通过吸附到纤维上从发酵液中除去。棉毛圈布表面粗糙，是细胞吸附的首选材料。细胞吸附棉比聚酯纤维快。吸附动力学可以通过一级速率方程建模。棉的吸附速率常数比涤纶高30-40%。细胞吸附是没有效率的黄原胶，这表明胞外多糖，黄原胶，是重要的有效细胞吸附到纤维。
Xanthan gum is a microbial polysaccharide widely used in food and oil‐drilling industries. Xanthan gum produced from the current commercial fermentation process usually contains cells and cell debris, which lower the filterability of the xanthan solution and limit its applications. The production of cell‐free xanthan gum fermentation broth is thus desirable. The feasibility of removing cells from the xanthan fermentation broth by cell adsorption to various woven fibrous materials was studied. It was found that both cotton and polyester fibers could be used to adsorb Xanthomonas campestris cells present in the fermentation broth either during batch fermentation or after the fermentation. Almost all cells were removed from the fermentation broth by adsorption to fibers. Cotton terry cloth had rough surfaces and was the preferred material for cell adsorption. Cell adsorption to cotton was faster than to polyester fibers. The adsorption kinetics can be modeled by a first‐order rate equation. The adsorption rate constants were 30–40% higher for cotton than for polyester. Cell adsorption was not efficient in the absence of xanthan gum, suggesting that the exopolysaccharide, xanthan gum, was important for efficient cell adsorption to fibers.