In vitro and in vivo Studies on the Antioxidant Effects of Soymilk Fermented with Streptococcus thermophilus grx02
In vitro and in vivo Studies on the Antioxidant Effects of Soymilk Fermented with Streptococcus thermophilus grx02
复制标题
嗜热链球菌grx02发酵豆奶抗氧化作用的体内外研究
DOI：
10.1080/08905436.2012.724039
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发表时间：
2012-01-01
影响因子：
1.8
通讯作者：
Gu, Ruixia
中科院分区：
文献类型：
--
作者：
Xu, Yin;Chen, Xia;Gu, Ruixia
评估了用嗜热链球菌 grx02 (FSM) 发酵的豆浆的抗氧化特性。 FSM 的体外抗氧化作用通过基于 2, 2-二苯基-1-三硝基苯肼 (DPPH) 的测定法进行测定，并确定对丙二醛 (MDA) 形成的抑制作用以及螯合亚铁离子的能力；各自的平均 IC50 值为 49.5、24.2 和 8.7 mg/mL。测定酒精喂养小鼠肝脏抗氧化酶活性、血清生化标志物和脂质过氧化指数，并比较ICR小鼠肝细胞的变化。结果显示，与对照组相比，阴性组丙氨酸转氨酶（ALT）活性、天冬氨酸转氨酶（AST）活性和甘油三酯（TG）升高（p < 0.05）。此外，FSM组血清ALT和AST活性低于阴性组（p < 0.05）。然而，血清中白蛋白（ALB）和总蛋白（TP）值（阳性组、阴性组、对照组和FSM组）没有显着差异（p>0.05）。与阴性组相比，FSM组肝脏中乙醇脱氢酶（ADH）、超氧化物歧化酶（SOD）活性和谷胱甘肽（GSH）含量升高（p < 0.05）。 FSM组肝脏MDA低于阴性组(p < 0.05)。
Soymilk fermented with Streptococcus thermophilus grx02 (FSM) was evaluated for its antioxidant properties. Antioxidant effects of FSM in vitro were measured with 2, 2-diphenyl-1-picrylhydrazyl (DPPH) based assay and by determining the inhibitory effect on the formation for malonyldialdehyde (MDA), and on the ability to chelate ferrous ions; the respective average IC50 values were 49.5, 24.2 and 8.7 mg/mL. Antioxidant enzymatic activities in liver of alcohol-fed mice, biochemical markers in serum and an index of lipid peroxidation were determined, and additionally the changes in hepatocytes of ICR mice were compared. The results showed that, compared with the control group, alanine transaminase (ALT) activity, aspartate transaminase (AST) activity and triglycerides (TG) of negative group increased (p < 0.05). Moreover, the serum ALT and AST activities of FSM group was lower than negative group (p < 0.05). However, there was no significant difference (p > 0.05) in the albumin (ALB) and total protein (TP) values (in positive, negative, control, and FSM groups) in the serum. Compared with the negative group, alcohol dehydrogenase (ADH), superoxide dismutase (SOD) activities and glutathione (GSH) content increased in liver (p < 0.05) in FSM group. The MDA of FSM group in liver was lower than the negative group (p < 0.05).