Quantitative image analysis of protein expression and colocalisation in skin sections
Quantitative image analysis of protein expression and colocalisation in skin sections
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皮肤切片中蛋白质表达和共定位的定量图像分析
DOI：
10.1111/exd.13457
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发表时间：
2018
影响因子：
3.6
通讯作者：
H. Törmä
中科院分区：
文献类型：
--
作者：
Hanqian Zhang;Maja Ericsson;M. Virtanen;S. Weström;Carolina Wählby;A. Vahlquist;H. Törmä
免疫荧光（IF）和原位邻位连接测定（isPLA）分别是用于原位蛋白表达和共定位分析的技术。然而，缺乏一种有效的定量方法来分析IF和isPLA染色的皮肤切片。因此，我们开发了一种新方法，使用免费开源软件CellProfiler半自动定量逐层测量皮肤切片中的蛋白质表达和共定位。作为原理证明，检查了鱼鳞病相关蛋白TGm-1和SDR 9 C7的IF和isPLA。结果表明，这种新方法可用于皮肤切片中的蛋白质表达和共定位分析。
Immunofluorescence (IF) and in situ proximity ligation assay (isPLA) are techniques that are used for in situ protein expression and colocalisation analysis, respectively. However, an efficient quantitative method to analyse both IF and isPLA staining on skin sections is lacking. Therefore, we developed a new method for semi‐automatic quantitative layer‐by‐layer measurement of protein expression and colocalisation in skin sections using the free open‐source software CellProfiler. As a proof of principle, IF and isPLA of ichthyosis‐related proteins TGm‐1 and SDR9C7 were examined. The results indicate that this new method can be used for protein expression and colocalisation analysis in skin sections.