Identification of Hsc70 as an influenza virus matrix protein (M1) binding factor involved in the virus life cycle
Identification of Hsc70 as an influenza virus matrix protein (M1) binding factor involved in the virus life cycle
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DOI：
10.1016/j.febslet.2006.09.040
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发表时间：
2006-10-16
期刊：
影响因子：
3.5
通讯作者：
Kobayashi, Nobuyuki
中科院分区：
文献类型：
--
作者：
Watanabe, Ken;Fuse, Takayuki;Kobayashi, Nobuyuki
流感病毒基质蛋白1（M1）在病毒复制、组装和出芽过程中起着重要作用。我们通过免疫沉淀和MALDI-TOF MS鉴定了热休克同源蛋白70（Hsc 70）作为M1结合蛋白。M1的C末端结构域与Hsc 70相互作用。我们发现Hsc 70与M1向细胞核的转运无关，但它确实抑制了M1和NP的核输出，从而抑制了病毒的产生。这是首次证明Hsc 70与M1直接相关，因此是病毒生产所必需的。(c)2006年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
Influenza virus matrix protein 1 (M1) has been shown to play a crucial role in the virus replication, assembly and budding. We identified heat shock cognate protein 70 (Hsc70) as a M1 binding protein by immunoprecipitation and MALDI-TOF MS. The C terminal domain of M1 interacts with Hsc70. We found that Hsc70 does not correlate with the transport of M1 to the nucleus, however, it does inhibit the nuclear export of M1 and NP, thus resulting in the inhibition of viral production. This is the first demonstration that Hsc70 is directly associated with M1 and therefore is required for viral production. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.