Effect of carbon source, C/N ratio, nitrate and dissolved oxygen concentration on nitrite and ammonium production from denitrification process by Pseudomonas stutzeri D6.
Effect of carbon source, C/N ratio, nitrate and dissolved oxygen concentration on nitrite and ammonium production from denitrification process by Pseudomonas stutzeri D6.
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DOI：
10.1016/j.biortech.2011.10.026
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发表时间：
2012
影响因子：
11.4
通讯作者：
Xin-ping Yang;Shimei Wang;Lixiang Zhou
中科院分区：
文献类型：
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作者：
Xin-ping Yang;Shimei Wang;Lixiang Zhou
利用从活性污泥中选择性分离的假单胞菌D6对反硝化过程中NO2-和NH4+的产生进行了研究。碳源类型、C/N比和氧气浓度的变化对反硝化过程中NO2-和NH4+的积累有显著影响。D6表现出对柠檬酸盐和醋酸盐的偏好，导致硝酸盐的还原数量最多，消耗最快，中间产物积累最少。结果表明，在较高的初始有机碳浓度或直接代谢碳型下，底物刺激提高了系统的耗氧率，可获得较完全的反硝化效果。培养物中氧气浓度越高，中间产物浓度越高。实验表明，硝酸盐浓度对NO2-和NH4+的产生影响不大。应优化生物脱氮系统，以促进完全反硝化，最大限度地减少NO2-和NH4+的积累。
Pseudomonasstutzeri D6, selectively isolated from activated sludge was used to study NO2-and NH4+production from denitrification processes. Changes in carbon type, C/N ratio and oxygen concentration significantly influenced the magnitude of NO2-and NH4+accumulation through denitrification. D6 showed a preference for citrate and acetate, which led to the largest quantity of nitrate reduced and which were exhausted most rapidly, with minimal intermediate products accumulation. It is found that at higher initial organic carbon concentration or for directly metabolic carbon type more complete denitrification could be obtained as a result of increase of the oxygen consumption rate by substrate stimulation. The higher the oxygen concentration in the culture was, the higher the intermediate products concentration became. The experiment showed that NO2-and NH4+production was only slightly influenced by nitrate concentration. Biological nitrogen removal systems should be optimized to promote complete denitrification to minimize NO2-and NH4+accumulation.