Interleukin-15 increases calcineurin expression in 3T3-L1 cells: Possible involvement on in vivo adipocyte differentiation
Interleukin-15 increases calcineurin expression in 3T3-L1 cells: Possible involvement on in vivo adipocyte differentiation
复制标题
DOI：
10.3892/ijmm_00000252
复制
发表时间：
2009-10-01
影响因子：
5.4
通讯作者：
Lopez-Soriano, Francisco J.
中科院分区：
文献类型：
--
作者：
Almendro, Vanessa;Fuster, Gemma;Lopez-Soriano, Francisco J.
不同的研究表明，Ca2+依赖性丝氨酸/苏氨酸磷酸酶钙调神经磷酸酶参与脂肪细胞分化的调节。钙调磷酸酶作为一种 Ca2+ 依赖性分子开关，通过阻止关键的促脂肪转录因子的表达来负向调节 3T3-L1 细胞进行脂肪细胞分化的能力。在这项研究中，我们研究了白细胞介素 15 (IL-15) 在 3T3-L1 前脂肪细胞系分化中的作用，白细胞介素 15 是一种细胞因子，之前已知参与控制脂肪细胞的脂肪堆积。我们发现，IL-15 能够增加长期接受细胞因子治疗的大鼠白色脂肪组织以及 3T3-L1 前脂肪细胞系中的 α-钙调神经磷酸酶 mRNA 含量。此外，根据 Red Oil 0 染色的估计，IL-15 促进瘦素 mRNA 表达和脂质积累的减少。与未治疗组相比，IL-15 和 FK506（一种钙调神经磷酸酶抑制剂）联合治疗后脂质含量没有变化。这些数据表明，IL-15 可能通过上调 α-钙调神经磷酸酶并阻止脂肪细胞分化的诱导来直接抑制脂肪生成。
Different studies have revealed that the Ca2+-dependent serine/threonine phosphatase calcineurin is involved in the regulation of adipocyte differentiation. Calcineurin acts as a Ca2+-dependent molecular switch that negatively regulates the ability of 3T3-L1 cells to undergo adipocyte differentiation by preventing the expression of critical proadipogenic transcription factors. In this study we investigated the role of interleukin-15 (IL-15), a cytokine previously known to be involved in the control of fat accretion by adipose cells, in the differentiation of the 3T3-L1 preadipose cell line. We found that IL-15 is able to increase a-calcineurin mRNA content in white adipose tissue of rats chronically treated with the cytokine and also in the 3T3-L1 preadipose cell line. Moreover, IL-15 promoted a decrease in both leptin mRNA expression and lipid accumulation, as estimated by Red Oil 0 staining. Cotreatment with IL-15 and FK506 (a calcineurin inhibitor) resulted in no changes in lipid content compared with the non-treated group. These data suggest that IL-15 directly inhibits adipogenesis, possibly by upregulating alpha-calcineurin and preventing the induction of adipocyte differentiation.